中华耳科学杂志2025，Vol.23Issue(7)：830-837,8.DOI:10.3969/j.issn.1672-2922.2025.07.003
基于非靶向代谢组学分析突发性耳聋血液标志物代谢特征
Metabolic Characteristics of Blood Markers of Sudden Deafness Based on Untargeted Metabolomics
摘要
Abstract
Objective To characterize metabolic profiles of potential biomarkers of sudden deafness by untargeted metabolomics.Methods Patients admitted to the Department of Otorhinolaryngology Head and Neck Surgery,the Second Affiliated Hospital of Army Medical University,between June 2022 and January 2023(n=30)were recruited,including 15 patients diagnosed with sudden sensorineural hearing loss(SSNHL)and 15 patients with simple deviated nasal septum and no hearing impairment.Venous blood samples were collected and subjected to untargeted metabolomics analysis using Liquid Chromatography-mass Spectrometry(LC-MS).Supervised Orthogonal Partial Least Squares Discriminant Analysis(OPLS-DA)was employed to identify significantly differential metabolites(variable importance in projection[VIP]>1,P<0.05),followed by pathway enrichment analysis.Results A total of 599 metabolites were identified in positive ion mode and 315 metabolites in negative ion mode.Quality control(QC)sample correlation analysis revealed R²values all approaching 1.OPLS-DA results indicated significant intergroup differences in metabolites.In positive ion mode,the OPLS-DA model demonstrated an R2Y of 0.974 and a Q2of 0.717,leading to the identification of 103 differential metabolites.In negative ion mode,the model showed an R2Y of 0.996 and a Q2of 0.571,with 65 differential metabolites identified.Enrichment analysis revealed significant clustering in 8 metabolic pathways.Pathway mapping analysis demonstrated elevated levels of phosphatidylcholine,O-acetylcholine,L-histidine,N-methylhistamine,phenylalanine,citric acid,and linoleic acid in the sudden deafness group compared to controls.Conversely,17α-hydroxyprogesterone,cortisol,androst-4-ene-3,17-dione,androgen,and 7-methylxanthine were found at lower concentrations in the sudden deafness group.Conclusion Lipid metabolism and amino acid metabolism may constitute principal underlying pathways involved in the metabolic dysregulation associated with SSNHL.The increase in the level of its metabolites may be one of the causes of sudden deafness.关键词
突发性耳聋/非靶向代谢组学/代谢标志物Key words
sudden deafness/untargeted metabolomics/metabolic markers引用本文复制引用
余维,覃泽平,朱先柏,谭国静,邹璨,邓安春..基于非靶向代谢组学分析突发性耳聋血液标志物代谢特征[J].中华耳科学杂志,2025,23(7):830-837,8.基金项目
重庆市自然科学基金(CSTB2022NSCQ-MSX0178) （CSTB2022NSCQ-MSX0178）
