中华耳科学杂志2025，Vol.23Issue(7)：863-868,6.DOI:10.3969/j.issn.1672-2922.2025.07.008
100dB SPL8～16kHz倍频带噪声暴露对小鼠听觉功能的影响
Effects of 100 dB SPL 8～16 kHz Octave Band Noise Exposure on Auditory Function in Mice
摘要
Abstract
Objective To report effects of 100 dB SPL 8～16 kHz octave band noise exposure on hearing,cochlear hair cells and synapses in adult CBA/CaJ mice.Methods CBA/CaJ mice with normal hearing at 16 weeks of age were grouped to be exposed to 8～16 kHz octave noise at 100 dB SPL for 7.5,15,30,60 or 120 min(n=5～7 in each group).Auditory brainstem response(ABR)and distortion product otoacoustic emission(DPOAE)thresholds were assessed at 1 day and 14 days after exposure.The cochlear basilar membrane was isolated for immunofluorescence staining,with hair cells and synapses quantified using the Amira software.Results At 1 d after noise exposure,ABR and DPOAE thresholds were significantly elevated in all groups,which would have basically recovered to normal levels at 14 days after noise exposure.Immunofluorescence showed no inner and outer hair cells loss,but significant inner hair cells synapses loss in a noise dose-dependent manner and more severe in those exposed for 60 or 120 min(about 50%in the 32～45 kHz region).Conclusion Exposure to 8～16 kHz octave noise at 100 dB SPL for 7.5 min may be sufficient to cause hidden hearing loss in mice,especially at high frequencies.For short exposure,synaptic loss precedes the loss of sensory hair cells and represents an early manifestation of noise damage.关键词
倍频噪声/暂时性阈移/耳蜗/隐性听力损失/耳蜗突触病Key words
octave noise/temporary threshold shift/cochlea/hidden hearing loss/cochlear synaptopathy引用本文复制引用
石科,李悦淇,王君,郭丹..100dB SPL8～16kHz倍频带噪声暴露对小鼠听觉功能的影响[J].中华耳科学杂志,2025,23(7):863-868,6.基金项目
河南省医学科技攻关计划省部共建项目(SBGJ202102055) （SBGJ202102055）
河南省科技攻关计划项目(222102310338) （222102310338）
河南省科技发展计划科技攻关项目(242102521022) （242102521022）
