中国兽药杂志2025，Vol.59Issue(9)：8-15,8.DOI:10.11751/ISSN.1002-1280.2025.9.02
三株动物检疫用Vero细胞的微卫星鉴定与分析
Identification and Analysis of Microsatellites in Three Strains of Vero Cells for Animal Quarantine
摘要
Abstract
To clarify the genetic characteristics and potential cross-contamination of three Vero cell lines preserved in the laboratory,this study employed microsatellite(STR)technology for molecular biological identification.DNA was extracted from the three Vero cell lines,followed by multiplex amplification of 10 loci,including 8 monkey/human-derived loci(such as D8S1106),1 rhesus monkey-specific locus(F13A01),and 1 human-specific locus(FGA).PCR products were detected and analyzed using an ABI 3730xl Genetic Analyzer.The results showed that the STR typing of two Vero cell lines fully matched the standard Vero cell line without contamination,while one cell line exhibited abnormal peaks at D19S245 and D6S1017 loci,indicating potential genetic variation.The STR identification method established herein can effectively verify the identity of Vero cells and detect contamination,providing technical support for constructing cell banks in animal quarantine.关键词
Vero细胞/STR/细胞鉴定/动物检疫Key words
Vero cells/STR/cell identification/animal quarantine分类
农业科技引用本文复制引用
张伟,邓丛良,汪琳,刘全国,安宁,吴京,徐立伟,高志强,史喜菊,赵相鹏,张佳玮,张小寒,白子龙,杜思乐,王素秋,蒲静,任彤..三株动物检疫用Vero细胞的微卫星鉴定与分析[J].中国兽药杂志,2025,59(9):8-15,8.基金项目
国家重点研发计划(2022YFD1800605) （2022YFD1800605）
中国海关科学技术研究中心自立项目(2023HZ06) （2023HZ06）
