中国兽药杂志2025，Vol.59Issue(9)：31-37,7.DOI:10.11751/ISSN.1002-1280.2025.9.05
超高效液相-串联质谱法检测黄芩解毒散中非法添加的9种抗菌药物
Detection of a Variety of Antibiotics Illegally Added to Huangqin Jiedu San by Ultra-high Performance Liquid Chromatography-tandem Massrometry
摘要
Abstract
A method was established using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry(UPLC-MS/MS)to detect the contents of nine antibacterial drugs,such as sulfadiazine,sulfamethoxazole,trimethoprim,perflurazone,amantadine,enrofloxacin,sarafloxacin,ciprofloxacin,and dalfloxacin mesylate in Huangqin Jiedu San.The samples were extracted by ultrasonic extraction using acetonitrile-0.2%formic acid aqueous solution(8∶2),then centrifuged and diluted with the initial mobile phase.AB 4000Q TRAP liquid chromatography-mass spectrometry was used for analysis,employing gradient elution and electrospray ionization positive mode with multiple reaction monitoring.The results showed a good linear relationship within the range of 2.0 to 50.0 ng/mL;the detection limit was 1 g/kg,and the quantitation limit was 2 g/kg.At three added concentrations of 0.2%,0.5%,and 1.0%,the recovery rates were between 95%～101%,with intra-batch and inter-batch variation coefficients all less than 10%.This method is sensitive,rapid,and has excellent parallel reproducibility,fully meeting the detection requirements.关键词
超高效液相色谱串联质谱法/黄芩解毒散/抗菌药物/非法添加Key words
ultra-high efficiency liquid chromatography tandem mass spectrometry(UPLC-MS/MS)/Scutellaria baicalensis detoxification powder/antimicrobial drugs/illegal additives分类
农业科技引用本文复制引用
门晓冬,魏秀丽,刘霄飞,张昊,刘莎莎,张传津,张志民,李有志..超高效液相-串联质谱法检测黄芩解毒散中非法添加的9种抗菌药物[J].中国兽药杂志,2025,59(9):31-37,7.基金项目
山东省重大科技创新工程项目家禽重要疫病防控与新药创制(2025CXGC010803) （2025CXGC010803）
