中山大学学报（自然科学版）（中英文）2025，Vol.64Issue(5)：1-10,10.DOI:10.13471/j.cnki.acta.snus.ZR20250017
GPR158四聚体结构初步解析
Preliminary structure determination of GPR158 tetramer
摘要
Abstract
GPR158,a member of class C GPCRs,has attracted much attention for its unique roles in neurotransmission and emotion regulation recently.The interaction between GPR158 and RGS7-Gβ5 complex has shown potential values in the pathological process of neuropsychiatric diseases such as depression.To better understand how GPR158 interacts with RGS 7-Gβ5,in the study,we coexpressed the GPR158-RGS 7-Gβ5 complex by using baculovirus expression system,and then purified for Cryo-EM sample preparation.During data processing,some GPR158 were found to form homotetramers and interacted with RGS7-Gβ5 unexpectedly,and a GPR158 homotetramer structure with a resolution of 0.422 nm was finally obtained.The structure shows that two pairs of GPR158 dimers are arranged side by side.Meanwhile,the structure of GPR158 tetramer,as the first one to be resolved in GPCR family,lays the foundation for subsequent structural research of GPCR oligomers.关键词
G蛋白偶联受体/GPR158/四聚体/冷冻电镜/结构解析Key words
GPCR/GPR158/tetramer/Cryo-EM/structure determination分类
生物科学引用本文复制引用
刘旋,范恒新,周宁坤,张影..GPR158四聚体结构初步解析[J].中山大学学报（自然科学版）（中英文）,2025,64(5):1-10,10.基金项目
广东省基础与应用基础研究基金(2020A1515110060) （2020A1515110060）
