植物研究2025，Vol.45Issue(5)：722-730,9.DOI:10.7525/j.issn.1673-5102.2025.05.007
组成型过表达PagPYL4基因对84K杨耐旱性与生长的影响
Effects of Constitutive Overexpression of PagPYL4 Gene on Drought Tolerance and Growth of 84K poplar
摘要
Abstract
In the context of climate change intensification and increasing limitation of arable land,improving drought tolerance in forest trees has emerged as a critical breeding goal.However,achieving enhanced stress resistance without compromising growth and biomass production remains a major challenge.In this study,transgenic 84K poplar(Populus alba×P.glandulosa'84K')lines constitutively overexpressing PagPYL4 gene,an abscisic acid(ABA)receptor gene,were developed,and their physiological and growth responses were evaluated under drought stress.The results showed that under the influence of exogenous ABA,the overexpression of PagPYL4 gene significantly altered stomatal aperture in plants.Under short-term drought treatment,compared with the wild type,the overexpression of PagPYL4 gene reduced water loss and improved drought tolerance;however,it also inhibited stomatal conductance and photosynthetic rate,leading to a significant decrease in growth rate of plant height and ground diameter.Our findings demonstrated that while PagPYL4 overexpression effectively enhanced drought resistance,its indiscriminate activation can impose substantial growth penalties.This highlighted the importance of spatially and temporally regulated gene expression strategies to balance stress resilience and biomass productivity in future tree breeding programs.关键词
84K杨/PagPYL4/耐旱/生长/气孔/光合作用Key words
Populus alba×P.glandulosa'84K'/PagPYL4/drought tolerance/growth/stomata/photosynthesis分类
农业科技引用本文复制引用
刘上,王锦华,哈斯,刘畅..组成型过表达PagPYL4基因对84K杨耐旱性与生长的影响[J].植物研究,2025,45(5):722-730,9.基金项目
黑龙江省自然科学基金项目(LH2024C050) （LH2024C050）
林木遗传育种全国重点实验室(东北林业大学)创新项目(2022A04) （东北林业大学）
中央高校基本科研业务费专项资金项目(2572024AW16). （2572024AW16）
