植物研究2025，Vol.45Issue(5)：731-744,14.DOI:10.7525/j.issn.1673-5102.2025.05.008
白桦BpDMPs基因家族生物信息学及BpDMP7功能分析
Bioinformatics Analysis of the BpDMPs Gene Family in Betula platyphylla and Exploration of the Function of BpDMP7 gene
摘要
Abstract
Birch(Betula platyphylla),as a perennial woody plant,is difficult to obtain homozygous plants and families due to its long growth cycle and complex genotype,which leads to slow progress in its breeding work.Double haploid breeding technology is a breeding method that has emerged in recent years.It only requires two generations to obtain homozygous plant families.Therefore,double haploid breeding technology can greatly shorten the process of forest breeding and obtain homozygous families,while the discovery and application of the DMP gene has opened up a new research direction in doubled haploid breeding.This article aimed to study the BpDMPs gene family in birch,and revealed its physicochemical properties,protein structure,systematic evolution,gene structure,chromosome distribution,and potential functions in the growth and development process of birch through bioinformatics analysis;to identify the birch DMP gene with haploid induction function in the gene family through expression pattern analysis,and verify the gene function through Arabidopsis complementation experiments.The bioinformatics analysis results showed that birch contained 14 gene family members,distributed on six chromosomes.Phylogenetic analysis showed that 14 genes in the BpDMPs gene family belonged to five branches.The predictive analysis of cis-acting elements in the promoter of the BpDMPs gene family showed that the promoter contained response elements related to growth and development,hormone metabolism,stress response,and so on.The qRT-PCR results showed that there were significant differences in the expression levels of BpDMPs in various tissues of birch,with BpDMP2 and BpDMP7 having higher expression levels in the stamens.Through Arabidopsis complementation experiments,it was demonstrated that the BpDMP7 gene can replenish the phenotype of Arabidopsis dmp8dmp9 mutant grain sterility.This article provided basic information for further studying the functions and regulatory mechanisms of the BpDMPs gene family,and offered a new perspective for understanding the roles these genes play in plant growth,development,and environmental adaptation processes.关键词
白桦/单倍体诱导系/DMP基因/生物信息学Key words
birch/haploid induction system/DMP gene/bioinformatics分类
农业科技引用本文复制引用
武思尧,王新宇,孙子腾,郑志民..白桦BpDMPs基因家族生物信息学及BpDMP7功能分析[J].植物研究,2025,45(5):731-744,14.基金项目
中国博士后科学基金第75批面上项目(2024M750382). （2024M750382）
