植物研究2025，Vol.45Issue(5)：745-754,10.DOI:10.7525/j.issn.1673-5102.2025.05.009
水稻OsBBTIs基因家族鉴定及在非生物胁迫下的表达分析
Identification of OsBBTIs Gene Family in Rice and Its Expression Analysis under Abiotic Stress
摘要
Abstract
Bowman-Birk trypsin inhibitor(BBTI)is a widely distributed trypsin inhibitor in plants,which plays an important role in the process of plant resistance to the stresses.In this study,11 rice BBTIs gene family members were identified at the genome level by bioinformatics methods,among which 10 OsBBTI genes were clustered at the front of chromosome 1,and OsBBTI13 gene was located on chromosome 3.OsBBTIs protein had N-terminal signal peptide and contained 1-3 typical conserved Bowman-Birk type domains.The promoter region of OsBBTIs gene had response elements related to plant hormones jasmonic acid,abscisic acid and the abiotic stresses.Transcriptome data showed that all OsBBTIs gene can be induced by jasmonic acid,and most OsBBTI genes can be expressed in response to the abiotic stresses.qRT-PCR was used to analyze the expression level of OsBBTIs gene under NaHCO3 and NaCl stresses,the results showed that the expression level of OsBBTIs gene increased under the saline-alkali stresses.This study laid a foundation for further study on the biological function of OsBBTIs proteins in abiotic stress.关键词
水稻/OsBBTIs基因/Bowman-Birk型结构域/非生物胁迫/表达分析Key words
Oryza sativa/OsBBTIs/BowB domain/abiotic stress/expression analysis分类
生物科学引用本文复制引用
卢禹彤,刘博妍,车仁祥,张玉坤,朱凤金,蓝兴国..水稻OsBBTIs基因家族鉴定及在非生物胁迫下的表达分析[J].植物研究,2025,45(5):745-754,10.基金项目
黑龙江省大学生创新创业训练计划项目(DC-2024267) （DC-2024267）
国家自然科学基金面上项目(31870300). （31870300）
