中医临床研究2025，Vol.17Issue(19)：18-24,7.DOI:10.3969/j.issn.1674-7860.2025.19.003
基于GEO和分子对接探究miRNA调控肝纤维化机制的研究
A study on the mechanism of miRNA regulating liver fibrosis based on GEO and molecular docking
摘要
Abstract
Objective:To explore the molecular mechanism of microRNA(miRNA)regulating hepatic fibrosis through bioinformatics analysis by the GEO database dataset.Methods:The data of GSE19865,GSE77271,and GSE66278 of miRNA microarrays of liver fibrosis mouse were obtained by the GEO database,and the differentially expressed genes of miRNAs were screened by the GEO 2R database software.The target genes were jointly predicted by two tools,microT-CDS and miRDB.The target genes were enriched and analyzed by Cytoscape plug-in ClueGO.A protein-protein interaction network analysis was performed on the target genes to obtain the key genes of liver fibrosis in the regulatory network,performing molecular docking with the core components for treating liver fibrosis.Results:The study revealed the identification of 101 miRNAs,of which 70 exhibited increased expression in liver fibrosis cells,while 31 displayed reduced expression.Predictive screening yielded eight miRNAs.Through gene ontology(GO)biological enrichment analysis,the main molecular functions including cellular response to hypoxia,negative regulation of natural killer cell chemotaxis,and cell development were obtained,as well as biological processes such as platelet-derived growth factor activated receptor activity,γ-aminobutyric acid receptor binding,and platelet-derived growth factor binding.The pro-apoptotic signals induced by Toll-like receptor cascade 10,Toll-like receptor cascade 5,and tumor necrosis factor receptor 1 were obtained by pathway enrichment analysis.Through protein-protein interaction network analysis,a total of 29 key genes,including RHOA,Fos proto-oncogene,inhibitor of nuclear transcription factor kappa B kinase regulatory subunit gamma,and NOTCH1,were screened out.The molecular docking results further demonstrated that RHOA,NOTCH1,SIRT1,APP,and CREB1 all had good binding capabilities with dihydrotanshinone I.Conclusion:mmu-miR-34a-5p,mmu-miR-802,mmu-miR-125a-5p,mmu-miR-15b,mmu-miR-342-3p,mmu-miR-379,and mmu-miR-450a-5p are all involved in the occurrence of hepatic fibrosis with the mechanism of targeting RHOA,NOTCH1,SIRT1 and other genes to regulate biological processes such as vascular endothelial agrowth factor.关键词
肝纤维化/微小核糖核酸/生物信息学/分子对接Key words
Liver fibrosis/miRNA/Bioinformatics/Molecular docking分类
医药卫生引用本文复制引用
赵靖宇,郑洋,罗淑娟..基于GEO和分子对接探究miRNA调控肝纤维化机制的研究[J].中医临床研究,2025,17(19):18-24,7.基金项目
广西中医药大学赛恩斯新医药学院大学生创新创业训练项目(自治区级)(S202213643033). （自治区级）
