中药新药与临床药理2025，Vol.36Issue(9)：1423-1431,9.DOI:10.19378/j.issn.1003-9783.2025.09.002
左金丸调节胃热证大鼠肠道菌群的机制
Study on the Modulation of Gut Microbiota by Zuojin Pills in Rats with Gastric Heat Syndrome
摘要
Abstract
Objective To investigate the effects of Zuojin Pills(ZJP)on gut microbiota in rats with gastric heat syndrome(GHS)using 16S rRNA high-throughput sequencing and elucidate its underlying mechanisms.Methods SD rats were randomly divided into three groups(n=6 per group):normal control,GHS model,and ZJP treatment.The model and ZJP groups received intragastric administration of capsaicin decoction(10 mL·kg-1,twice daily)for 6 days.From day 4,the ZJP group was additionally given ZJP aqueous extract(3.5 g·kg-1,once daily)for 4 days,while other groups received equal volumes of distilled water.On day 7,after 24-hour fasting,the model and ZJP groups were administered anhydrous ethanol(1.5 mL),and the normal group received distilled water.Gastric histopathology was assessed via HE staining;serum interleukin-2(IL-2),interleukin-8(IL-8),and gastric tissue prostaglandin E2(PGE2)and epidermal growth factor(EGF)levels were measured by ELISA;gut microbiota composition was analyzed via 16S rRNA sequencing.Results Compared with the normal group,the model group exhibited dark red gastric mucosa with uneven surfaces,capillary dilation,congestion,edema,and patchy/linear hemorrhages;histopathology revealed epithelial necrosis,glandular disarray,inflammatory infiltration,and erythrocyte extravasation.Serum IL-2 and IL-8 levels significantly increased(P＜0.01),while gastric PGE2 and EGF levels decreased(P＜0.01).Gut microbiota analysis showed elevated Simpson index(P＜0.05)and reduced Shannon index(P＜0.05),with significant shifts in composition:decreased relative abundances of Bacteroidetes and Verrucomicrobia phyla,and altered abundances of 11 genera(e.g.,increased Prevotella_1,Clostridium_innocuum_group,Streptococcus,Morganella,Klebsiella;decreased Defluviitaleaceae_UCG_011,Eubacterium_ventriosum_group,Lachnospiraceae_UCG_001,Mobilitalea,Eubacterium_xylanophilum_group,Ruminococcus_1).Comparison with the model group,rats in the ZJP group exhibited only scattered punctate or linear hemorrhages in gastric mucosa with significant macroscopic improvement.The ZJP group showed only focal gastric hemorrhages and mild inflammation,with significantly improved pathology.Serum IL-2 and IL-8 levels decreased(P＜0.01,P＜0.05),while gastric PGE2 and EGF levels increased(P＜0.01).Gut microbiota ACE,Chao1,and Shannon indices decreased(P＜0.05),with reversal of 6 key genera(Defluviitaleaceae_UCG_011,Mobilitalea,Ruminococcus_1,Streptococcus,Morganella,Klebsiella)and enrichment of Proteus,Eubacterium_fissicatena_group,Erysipelatoclostridium,Parasutterella,Prevotellaceae_NK3B3 1_group,Bacteroides,alongside reduced Butyricimonas,Odoribacter,and Tyzzerella.Conclusion ZJP may ameliorate GHS by suppressing inflammatory cytokines and restoring gut microbiota homeostasis.关键词
左金丸/胃热证/16S rRNA高通量测序/肠道菌群/炎症因子/大鼠Key words
Zuojin Pills/gastric heat syndrome/16S rRNA sequencing/gut microbiota/inflammatory cytokines/rats分类
医药卫生引用本文复制引用
张玉杰,张璐,杨静,邹忠杰,龚梦鹃..左金丸调节胃热证大鼠肠道菌群的机制[J].中药新药与临床药理,2025,36(9):1423-1431,9.基金项目
国家自然科学基金项目(81403075). （81403075）
