遵义医科大学学报2025，Vol.48Issue(9)：889-895,7.
金钗石斛生物碱对小鼠肠道菌群的作用
Effects of Dendrobium nobile Lindl.alkaloids on gut microbiota in mice
摘要
Abstract
Objective This study explores the effect of Dendrobium nobile Lindl.alkaloids(DNLA)on the gut microbiota of normal mice.Methods Kunming mice were randomly assigned to the normal control group(Con-trol),the low-dose DNLA group(15 mg/kg),and the high-dose DNLA group(30 mg/kg),and were adminis-tered orally for 7 days.The bacteria DNA was extracted,and 16S rRNA sequencing was performed.Moreover,the intestinal bacteria genes were verified by RT-qPCR with specific microbiome primers.Results High dose of DNLA increased Firmcutes/Bacteriodetes(40％)at the Phylum level;DNLA also increased clostridiaceae,de-halobacteriaceae,enterobacteriaceae,odoribacteraceae and streptococcaceae,etc.at the family level;at the ge-nus level,DNLA increased Prevotella;at the species/gene level,DNLA increased R.bacterium,Prevotella,Blautia coccoide and Bacteroides fragilis and decreased Fusobacterium nucleatum and Clostridium coccoides(P＜0.05).Conclusion DNLA could affect gut microbiota in normal mice,which could be important for DNLA to exert beneficial effects through microbiota-gut-brain axis,or microbiota-gut-liver axis.关键词
金钗石斛生物碱/小鼠/肠道菌群/16S rRNA测序/RT-qPCRKey words
Dendrobium nobile Lindl alkaloids/mice/gut microbiota/16S rRNA-Seq/RT-qPCR分类
医药卫生引用本文复制引用
徐云燕,刘杰,刘波..金钗石斛生物碱对小鼠肠道菌群的作用[J].遵义医科大学学报,2025,48(9):889-895,7.基金项目
贵州省中医药管理局中医药、民族医药科学技术研究基金资助项目(NO:QZYY2017-009) （NO:QZYY2017-009）
遵义医科大学基础药理教育部重点实验室主任基金资助项目(NO:JCYL-Z-003). （NO:JCYL-Z-003）
