中医正骨2025，Vol.37Issue(8)：4-9,6.
畲药祛邪补肾汤治疗踝关节创伤后骨关节炎作用机制的实验研究
Mechanism of She drug Quxie Bushen Tang(祛邪补肾汤)in treatment of post-traumatic osteoarthritis of an-kle:an experimental study
摘要
Abstract
Objective:To investigate the mechanism of She drug Quxie Bushen Tang(祛邪补肾汤,QXBST)in treatment of post-trau-matic osteoarthritis(PTOA)of ankle.Methods:Eighteen 12-week-old healthy male C57BL/6J mice were selected and randomized into sham-operated group,model group,and QXBST group,with 6 ones in each group.All mice but the ones in sham-operated group were subjec-ted to surgeries for building PTOA models by transecting the anterior talofibular ligament(ATFL)of the right hindlimb ankle,while the ones in sham-operated group were merely incised the skin at the corresponding site,without transecting ATFL.Twenty-four hours after the model-ing surgery,the mice in QXBST group were intervened by intragastric administration with 0.4 mL QXBST decoction(crude drug dosage:1.63 g/mL),while the ones in model group and sham-operated group with an equal volume of normal saline,once a day for consecutive 12 weeks.On day 2 after the end of intragastric administration,all mice were sacrificed by cervical dislocation,and their right hindlimb ankle joints were harvested to make specimens for analysis.The ankle joint specimens were scanned by using a Micro-CT scanner for analyzing the subchondral bone microarchitecture such as bone volume/tissue volume(BV/TV)and trabecular separation(Tb.Sp),and the ankle joint cartilage degeneration was assessed using alcian blue-hematoxylin-orange G(ABH-OG)staining,and toluidine blue staining,respectively.Moreover,the ankle articular cartilage area was measured using a bone histomorphometry analysis and measurement system,and the cartilage degeneration severity was graded using the Osteoarthritis Research Society International(OARSI)semi-quantitative scoring system.Further-more,the expression levels of collagen type Ⅱ(COL2)and tumor necrosis factor-α(TNF-α)in the ankle cartilage tissues were detected by u-sing immunohistochemical staining.Results:①Subchondral bone microarchitecture.The QXBST group exhibited greater BV/TV,and lower Tb.Sp compared to model group(P=0.000,P=0.002);while,compared to sham-operated group,the QXBST group exhibited greater BV/TV,with no significant difference observed in Tb.Sp(P=0.000,P=0.284).②Cartilage degeneration.The QXBST group showed larger cartilage area and lower OARSI score compared to model group(P=0.000,P=0.000),while the QXBST group was comparable to the sham-operated group in both cartilage area and OARSI score(P=0.542,P=0.401).③Expression levels of COL2 and TNF-α in the ankle cartilage tissues.The QXBST group demonstrated increased COL2-positive expression area and a lower proportion of TNF-α-positive chondro-cytes compared to model group(P=0.000,P=0.001);while,compared to the sham-operated group,the QXBST group exhibited a higher pro-portion of TNF-α-positive chondrocytes,with no significant difference observed in COL2-positive expression area(P=0.000,P=0.216).Conclusion:She drug QXBST can improve the ankle subchondral bone microarchitecture,and attenuate ankle cartilage degeneration in the an-kle PTOA model mice.It may work by up-regulating the level of COL2 and down-regulating the level of TNF-α in the ankle cartilage tissues.关键词
骨关节炎/踝损伤/软骨,关节/畲药/胶原Ⅱ型/肿瘤坏死因子-α/动物实验/小鼠/祛邪补肾汤Key words
osteoarthritis/ankle injuries/cartilage,articular/She drugs/collagen type Ⅱ/tumor necrosis factor-alpha/animal experimenta-tion/mice/Quxie Bushen Tang引用本文复制引用
邹光翼,李明亮,梁伟..畲药祛邪补肾汤治疗踝关节创伤后骨关节炎作用机制的实验研究[J].中医正骨,2025,37(8):4-9,6.基金项目
浙江省中医药优秀青年人才基金项目(2021ZQ091) （2021ZQ091）
浙江省医药卫生科技计划项目(2021PY032) （2021PY032）
