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CRISPR-based shuttle cloning of 1397 human genes into UAS vectors

Xuelian Liu Hanqing Xi Miao Dai Xiaoxue Li Wen Xue Guang Chen Jialong Yan Si Xu Guifang Ou Si Luo Yonghong Tang Ping Wei Jiwu Wang

Acta Biochimica et Biophysica Sinica2025，Vol.57Issue(8)：P.1376-1380,5.

Acta Biochimica et Biophysica Sinica2025，Vol.57Issue(8)：P.1376-1380,5.DOI:10.3724/abbs.2025050

CRISPR-based shuttle cloning of 1397 human genes into UAS vectors

Xuelian Liu ¹Hanqing Xi ²Miao Dai ²Xiaoxue Li ³Wen Xue ¹Guang Chen ¹Jialong Yan ¹Si Xu ²Guifang Ou ²Si Luo ⁴Yonghong Tang ²Ping Wei ⁴Jiwu Wang¹

作者信息

1. Clinical Research Institute,the Affiliated Nanhua Hospital,Hengyang Medical School,University of South China,Hengyang 421002,China
2. Clinical Research Institute,the Affiliated Nanhua Hospital,Hengyang Medical School,University of South China,Hengyang 421002,China Department of Neurology,the Affiliated Nanhua Hospital,Hengyang Medical School,University of South China,Hengyang 421002,China
3. Clinical Research Institute,the Affiliated Nanhua Hospital,Hengyang Medical School,University of South China,Hengyang 421002,China Institute of Biochemistry and Molecular Biology,Hengyang Medical School,University of South China,Hengyang 421002,China
4. Shanghai Diabetes Institute,Shanghai Key Laboratory of Diabetes Mellitus,Shanghai Clinical Center for Diabetes,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200233,China
折叠

摘要

关键词

CRISPR/dna library/inverted repeats/elucidating function all genes/UAS vectors/functional genomics/cdnasorfsgene promoters/shuttle cloning

分类

生物科学

引用本文复制引用

Xuelian Liu,Hanqing Xi,Miao Dai,Xiaoxue Li,Wen Xue,Guang Chen,Jialong Yan,Si Xu,Guifang Ou,Si Luo,Yonghong Tang,Ping Wei,Jiwu Wang..CRISPR-based shuttle cloning of 1397 human genes into UAS vectors[J].Acta Biochimica et Biophysica Sinica,2025,57(8):P.1376-1380,5.

基金项目

supported by the grants from the National Natural Science Foundation of China(No.32071135) （No.32071135）

startup fund from the Affiliated Nanhua Hospital,Hengyang Medical School,University of South China. （）

Acta Biochimica et Biophysica Sinica

ISSN：1672-9145

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