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首页|期刊导航|Acta Biochimica et Biophysica Sinica|CRATS:a one-pot CRISPR-Cas12b and RPA combined assay with a temperature switchcong for highly sensitive detection of monkeypox virus

CRATS:a one-pot CRISPR-Cas12b and RPA combined assay with a temperature switchcong for highly sensitive detection of monkeypox virus

Guangxi Yu Yue Wang Yukang Chen Jiangyuan Liu Hongtao Kang Xiaodong Luan Song Gao Pei Wang

Acta Biochimica et Biophysica Sinica2025,Vol.57Issue(8):P.1371-1375,5.
Acta Biochimica et Biophysica Sinica2025,Vol.57Issue(8):P.1371-1375,5.DOI:10.3724/abbs.2025016

CRATS:a one-pot CRISPR-Cas12b and RPA combined assay with a temperature switchcong for highly sensitive detection of monkeypox virus

Guangxi Yu 1Yue Wang 1Yukang Chen 1Jiangyuan Liu 1Hongtao Kang 1Xiaodong Luan 2Song Gao 1Pei Wang3

作者信息

  • 1. Jiangsu Key Laboratory of Marine Biological Resources and Environment,Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Ocean University,Lianyungang 222005,China
  • 2. Jiangsu Key Laboratory of Marine Biological Resources and Environment,Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Ocean University,Lianyungang 222005,China School of Electronic Engineering,Jiangsu Ocean University,Lianyungang 222005,China
  • 3. School of Marine and Biological Engineering,Yancheng Teachers University,Yancheng 224007,China
  • 折叠

摘要

关键词

cleave chemically labelled molecular beacons/one pot/crispr cas b/guide rna/detection targets/detectionscas nucleases/nucleic acid/molecular detectionsespecially

分类

医药卫生

引用本文复制引用

Guangxi Yu,Yue Wang,Yukang Chen,Jiangyuan Liu,Hongtao Kang,Xiaodong Luan,Song Gao,Pei Wang..CRATS:a one-pot CRISPR-Cas12b and RPA combined assay with a temperature switchcong for highly sensitive detection of monkeypox virus[J].Acta Biochimica et Biophysica Sinica,2025,57(8):P.1371-1375,5.

基金项目

supported by the grants from the National Natural Science Foundation of China(Nos.62104087 and 31470275) (Nos.62104087 and 31470275)

the China Postdoctoral Science Foundation(Nos.2024M751207 and 2022M721665) (Nos.2024M751207 and 2022M721665)

the Jiangsu Provincial Natural Science Foundation of China(No.BK20241088) (No.BK20241088)

the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(No.24KJB180026) (No.24KJB180026)

the Research Program of the“521 Project”of Lianyungang City of China(No.LYG06521202133) (No.LYG06521202133)

the Open-end Funds of Jiangsu Key Laboratory of Marine Bioresources and Environment(Nos.SH20221208 and SH20231212) (Nos.SH20221208 and SH20231212)

the Postgraduate Research&Practice Innovation Program of Jiangsu Province(Nos.SJCX23_1837,SJCX23_1839,SJCX24_2097 and SJCX24_2098) (Nos.SJCX23_1837,SJCX23_1839,SJCX24_2097 and SJCX24_2098)

the Priority Academic Program Development of Jiangsu Higher Education Institutions of China。 ()

Acta Biochimica et Biophysica Sinica

1672-9145

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