癌变·畸变·突变2025，Vol.37Issue(5)：400-404,5.DOI:10.3969/j.issn.1004-616x.2025.05.009
全血接种量及培养体系对淋巴细胞核质桥分析的影响
Effect of whole blood inoculum and culture system on lymphocyte nucleoplasmic bridges formation
摘要
Abstract
OBJECTIVE:To investigate effects of whole blood inoculation and culture systems on formation of nucleoplasmic bridges(NPB)and on formation of radiation-induced NPB,for providing scientific basis for radiation biological dosimeters.METHODS:Human venous blood and fingertip blood were cultured as follows:400,300,200,100 and 50 μL of venous blood were inoculated into 1 or 2 mL medium,100 μL of fingertip blood were inoculated into 1 mL medium.These cultures were irradiated with 0 Gy(control group)and 2 Gy 60Co γ radiation at a dose rate of 1.0 Gy/min.NPB specimens were prepared by cytokinesis-block method.The proportion of mononucleated,binucleated and multinucleated cells,as well as the NPB and micronucleus(MN)frequencies of each group were analyzed.RESULTS:In the 0 Gy group,1 048～9 768 binucleated cells were analyzed in different blood inoculation groups.The NPB frequency did not change significantly with the whole blood inoculation(P>0.05).The MN frequency in the 50 μL group was higher than that in the other three groups(U=3.61,P<0.01).In the 2 Gy group,there was no cell available for analysis in the 50 μL group,and 1 569-4 400 binucleated cells were analyzed in other blood inoculation groups.The NPB frequency was 0.009-0.013 cells/cell,and the MN frequency was 0.317-0.337 cells/cell,and there was no significant difference with the whole blood inoculum(P>0.05).The NPB and MN frequencies of the 2 Gy group were significantly higher than those of the 0 Gy group(U=4.24-34.48,P<0.05).Fingertip blood culture failed to observe cells for analysis.CONCLUSION:The minimum 50 μL and 100 μL peripheral blood culture combined with 1 mL culture medium can be used to study the background level of the nucleoplasmic bridge and the radiation-induced nucleoplasmic bridge level respectively.The feasibility of fingertip blood culture for nucleoplasmic bridge analysis still needs further study.关键词
核质桥/全血接种量/胞质分裂阻滞/微核Key words
nucleoplasmic bridge/whole blood inoculum/cytokinesis-block/micronucleus分类
医药卫生引用本文复制引用
赵骅,陆雪,蔡恬静,齐雪松,刘青杰..全血接种量及培养体系对淋巴细胞核质桥分析的影响[J].癌变·畸变·突变,2025,37(5):400-404,5.基金项目
国家自然科学基金(82003393) （82003393）
中国疾病预防控制中心辐射安全所青年科学研究所长基金(2023-02) （2023-02）
