安徽农业科学2025，Vol.53Issue(19)：24-30,7.DOI:10.3969/j.issn.0517-6611.2025.19.005
基于分子标记的辣椒种质资源遗传多样性及群体结构分析
Analysis of Genetic Diversity and Population Structure of Pepper Germplasm Resources Based on Molecular Markers
摘要
Abstract
To explore the genetic background of five types of pepper germplasm resources and select core breeding materials,this study used molecular markers to analyze their genetic diversity and population structure.The research results showed that 24 pairs of markers amplified 55 alleles from 115 materials,with 2.29 for each pair of primers in average.The range of allele frequency variation was 0.49-0.97,with an av-erage of 0.75;the variation range of genetic diversity(GD)is 0.05-0.65,with an average of 0.33.Among them,the GD value of the goat horn pepper type is the highest,at 0.65,and the heterozygosity variation range is 0-0.99.The PIC values of primers CIDH203 and P52-11-21 are greater than 0.5,indicating that these two pairs of markers are highly polymorphic primers with high amplification polymorphism in all samples.UPGMA clustering based on Nei's genetic distance divides these germplasm resources into three different subgroups,Group 1,Group 2,and Group 3.Population structure analysis reveals that when K=4,the tested materials can be divided into four different subgroups.This study revealed the genetic background of five types of pepper germplasm resources,screened high polymorphism primers and core breed-ing materials,and provided theoretical basis and practical guidance for targeted breeding and genetic improvement of pepper varieties.关键词
辣椒/种质资源/分子标记/遗传多样性/群体结构Key words
Pepper/Germplasm resources/Molecular markers/Genetic diversity/Population structure分类
农业科技引用本文复制引用
卢霞,阿门,赵玉虎,刘圆芝,许光利,李文虎,张文婷..基于分子标记的辣椒种质资源遗传多样性及群体结构分析[J].安徽农业科学,2025,53(19):24-30,7.基金项目
江苏省现代农业(蔬菜)产业技术体系(JATS[2018]188). （蔬菜）
