草业学报2025，Vol.34Issue(10)：120-131,12.DOI:10.11686/cyxb2024472
氮肥减量配施腐植酸对北疆滴灌玉米田土壤真菌群落的影响
Effects of nitrogen fertilizer reduction combined with humic acid on soil fungal communities in drip irrigated maize fields in northern Xinjiang
摘要
Abstract
The objective of this study was to evaluate the effect of humic acid addition on soil fungal communities in drip irrigated maize(Zea mays)fields in northern Xinjiang under reduced nitrogen fertilizer supply,and to explore the optimal application of nitrogen fertilizer in farmland.In this study,a randomized block experiment was adopted,and six treatments were set up:No nitrogen fertilizer(CK,0 kg·ha-1),conventional nitrogen fertilizer(T1,300 kg·ha-1),humic acid application alone(T2,90 kg·ha-1),conventional nitrogen fertilizer combined with humic acid(T3),nitrogen fertilizer reduction of 15%combined with humic acid(T4),and nitrogen fertilizer reduction of 30%with humic acid(T5).Illumina MiSeq high-throughput sequencing technology was used to analyze the changes in fungal diversity and community structure under the six different treatments,and to explore the effects of nitrogen fertilizer reduction combined with humic acid application on soil fungal community composition.Supporting data on soil nutrient levels and maize yield were also collected.It was found that the soil electrical conductivity(EC)of T4 and T5 was significantly reduced by 18.35%and 24.85%,respectively,compared with T1,and the soil alkaline hydrolyzable nitrogen content was significantly increased by 32.00%and 18.40%,respectively under the same treatments.The maize yield was highest(18038.75 kg·ha-1)in the T4 treatment;however,the treatments had no significant effects on the soil fungal community Alpha and Beta diversity.With respect to soil fungi,the relative abundance of Ascomycota under T4 and T5 was significantly increased by 21.85%and 24.59%,respectively,compared with T2,but the relative abundance of Mortierellomycota and Chytridiomycota was significantly reduced.Pearson correlation analysis showed that soil conductivity was significantly positively correlated with Ascobolus and negatively correlated with Botryotrichum,while soil organic matter was negatively correlated with Fusarium presence,and soil alkaline hydrolyzable nitrogen was significantly positively correlated with Cladosporum presence.Further redundancy analysis showed that soil available phosphorus was the main environmental factor affecting the community structure of fungi in this drip irrigated maize field,while soil EC value and alkali hydrolyzable nitrogen were the main environmental factors affecting the horizontal community structure of soil fungi.In conclusion,nitrogen fertilizer reduction combined with humic acid changed the soil fungal community structure,promoted the growth of beneficial bacteria,and inhibited the reproduction of pathogenic fungi by causing changes in soil nutrient content.This information is of great significance for improving the soil microbial environment,maintaining the balance of the soil microbial community and increasing crop yield.关键词
玉米/氮肥/腐植酸/真菌群落/土壤养分Key words
corn/nitrogenous fertilizer/humic acid/fungal communities/soil nutrients引用本文复制引用
卢小倩,陈金露,杨卫君,郭青云,王单丽,赵红梅..氮肥减量配施腐植酸对北疆滴灌玉米田土壤真菌群落的影响[J].草业学报,2025,34(10):120-131,12.基金项目
自治区"三农"骨干人才培养项目(2024SNGGGCC020),新疆土壤与植物生态过程重点实验室资助项目(23XJTRZW10)和自治区天山英才-青年拔尖人才项目(2023TSYCCX0085)资助. （2024SNGGGCC020）
