草业学报2025，Vol.34Issue(10)：187-201,15.DOI:10.11686/cyxb2024424
白三叶几丁质酶基因家族鉴定及TrChit3功能分析
Identification of the chitinase gene family and functional analysis of TrChit3 from white clover
摘要
Abstract
Chitinases are glycoside hydrolases that play an important role in plant growth and development.Relatively few studies have focused on the responses of chitinases to various biotic and abiotic stresses in white clover(Trifolium repens).Therefore,in this study,we searched the whole genome of white clover to identify and characterize its chitinase gene family.A total of 44 chitinase genes were identified in the whole genome of white clover,and they were unevenly distributed on 16 chromosomes.Phylogenetic analysis of these chitinase genes classified them into two mega-groups(GH18 and GH19),with five separate clusters(I-V).Twenty-nine chitinase genes belonged to the GH18 subfamily and 15 belonged to the GH19 subfamily.The genes within the same subfamily genes encoded proteins with similar structures and functions.Promoter analysis showed that the promoter regions of these 44 chitinase genes contained a variety of cis-acting elements related to growth and development,hormones,and stress.Furthermore,quantitative reverse-transcription polymerase chain reaction analyses showed that salt stress and drought stress resulted in significant increases in the transcript levels of chitinase genes,which further verified their roles in the plant stress response.TrChit3 was selected for a detailed analysis of its role in drought resistance.Overexpression of TrChit3 in Arabidopsis thaliana enhanced its resistance to drought stress,as measured by changes in physiological indexes such as relative conductivity and malondialdehyde content.These results lay a foundation for further studies on the roles of chitinases in plants.关键词
白三叶/几丁质酶/基因家族/表达分析Key words
white clover/chitinase/gene family/expression analysis引用本文复制引用
秦晓芳,何芷睿,贾彤,杨玉娇,付薇,李航,彭燕..白三叶几丁质酶基因家族鉴定及TrChit3功能分析[J].草业学报,2025,34(10):187-201,15.基金项目
四川省饲草创新团队项目(No.SCCXTD-2024-16)资助. （No.SCCXTD-2024-16）
