草业科学2025，Vol.42Issue(9)：2244-2251,8.DOI:10.11829/j.issn.1001-0629.2025-0075
冰草高蛋白含量相关EST-SSR标记开发与验证
Development and validation of EST-SSR markers associated with high protein content in wheatgrass
摘要
Abstract
Crude protein content is one of the important nutritional quality traits for wheatgrass.To obtain the EST-SSR markers which are closely related to crude protein content,we used the F2 populations of a tetraploid crested wheatgrass which was generated by doubling the hybrid genome of Agropyron mongolicum and A.cristatum,and then used bulked segregant analysis(BAS)technology to develop EST-SSR marks.A total of 42 pairs of EST-SSR primers were finally amplified by polymerase chain reaction(PCR)to develop EST-SSR markers related with high protein content in wheatgrass.The F2 segregation individuals were used for validation and correlation analysis of marker sites.The results showed that the EST-SSR marker SSR-BC5-82 was closely correlated with crude protein content of wheatgrass.This EST-SSR marker can significantly and accurately mark crude protein content,and will be important in breeding new wheatgrass germplasm with high protein content and in enriching the molecular marker types of wheatgrass.关键词
冰草/高蛋白含量/EST-SSR标记/开发/验证Key words
wheatgrass/high protein content/EST-SSR markers/development/validation引用本文复制引用
杨东升,刘雨雷,王海伟,苏志芳,王海燕,郭利平,安琪,郝水源..冰草高蛋白含量相关EST-SSR标记开发与验证[J].草业科学,2025,42(9):2244-2251,8.基金项目
内蒙古自治区自然科学基金面上项目(2023MS03049) （2023MS03049）
河套学院高层次人才引进科研启动经费资助项目(HYRC202310) （HYRC202310）
内蒙古自治区"科技兴蒙"行动重点专项(NMKJXM202201) （NMKJXM202201）
巴彦淖尔市科技计划项目(NMKJXM202408) （NMKJXM202408）
