福建农业学报2025，Vol.40Issue(7)：662-668,7.DOI:10.19303/j.issn.1008-0384.2025.07.003
部分去除原生殖细胞对红鲫性别分化的影响
Effect of Inducible Primordial Germ Cell Removal on Altering Sexuality of Red Crucian Carp(Carassius auratus red var.)
摘要
Abstract
[Objective]A primordial germ cell(PGC)-removed red crucian carp(Carassius auratus red var.)was obtained to investigate effects of the genetic alternation on the sexual development and reproduction of the fish.[Method]A PGC-specific expression of nitroreductase(Ntr)-containing strain of red crucian carp line with PGC removed by a metronidazole(Mtz)immersion-targeted treatment at early development stage was continuously observed,sampled,and dissected for anatomical differentiations under a microscope.[Result]A stable and heritable red crucian carp line carrying the specific PGC marker fluorescence was successfully secured.Since Mtz can be converted by Ntr into a cytotoxic metabolite,the induction in the fish did not affect the PGC migration nor the number of fluorescently labeled cells.Thus,the PGC-removed transgenic line showed no pathological changes in the gonads and continued to reproduce offsprings with a sex ratio similar to that of control.[Conclusion]The removal of PGCs in red crucian carp at the embryonic stage exhibited no interference to the sexual development of the fish,and the sex differentiation of this fish may depends on the somatic cells of gonads.关键词
红鲫/原生殖细胞/条件性去除/性别分化Key words
Carassius auratusred var./primordial germ cell/inducible removal/sex differentiation分类
生物科学引用本文复制引用
王芳,欧密,张新铖,刘海洋,罗青,费树站,赵建,李凯彬..部分去除原生殖细胞对红鲫性别分化的影响[J].福建农业学报,2025,40(7):662-668,7.基金项目
国家重点研发计划(2022YFF0710502) （2022YFF0710502）
