福建农业学报2025，Vol.40Issue(7)：706-715,10.DOI:10.19303/j.issn.1008-0384.2025.07.008
多粘类芽孢杆菌NPDY05-8的全基因组序列分析
Whole Genome Sequence of Paenibacillus polymyxa NPDY05-8
摘要
Abstract
[Objective]Genome sequence and functions of Paenibacillus polymyxa NPDY05-8 were studied to explore the application potential of the bacterium as a biocontrol agent for wheat fusarium head blight and corn stem rot.[Methods]Antimicrobial spectrum of P.polymyxa NPDY05-8 was analyzed by the plate confrontation method,sequence and assembly obtained based on the Nonopore third-generation and Illumina second-generation sequencing technology platforms,and gene functions predicted and annotated.[Results]P.polymyxa NPDY05-8 showed antagonistic activities against 19 pathogenic fungi with antimicrobial spectra between 62.00%and 92.00%.The whole genome was 5132538 bp with a G+C content of 45.63%.There were 5151 genes and 4949 protein coding sequences predicted that included a plasmid of 226178 bp.The COG,GO,and KEGG databases annotated 3902,3489,and 1391 genes,respectively.A total of 209 carbohydrate-related enzymes and 11 secondary metabolite gene clusters were predicted.[Conclusion]P.polymyxa NPDY05-8 was rich in carbohydrate-related enzymes and contained 4 cellulase genes and synthetic secondary metabolites including several antimicrobial compounds,such as fusaricidi B,surfacetin,paenicidin B,bacitracin,aurantin B,C and D.It appeared to have significant potential for developing as a biocontrol agent.关键词
多粘类芽孢杆菌/全基因组/碳水化合物相关酶/次级代谢产物/杀镰刀菌BKey words
Paenibacillus polymyxa/whole-genome/carbohydrate-related enzymes/secondary metabolites/Fusaricidi B分类
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邓云,陈泳和,陈春花,谢冬容,刘端华,饶溶晖,苏妍,许卿,阮宏椿..多粘类芽孢杆菌NPDY05-8的全基因组序列分析[J].福建农业学报,2025,40(7):706-715,10.基金项目
福建省科技计划引导性项目(2024N0046) （2024N0046）
福建省农业科学院英才项目(YC2019004) （YC2019004）
