分析测试学报2025，Vol.44Issue(10)：2191-2197,7.DOI:10.12452/j.fxcsxb.241203571
负载HRP的纳米复合酶用于比色法测定牡蛎中的次黄嘌呤
HRP-loaded Nanocomposite Enzyme for Colorimetric Determination of Hypoxanthine in Oysters
摘要
Abstract
In this study,we have constructed Fe3O4@ZIF-8@HRP@SiO2 nanocomposite materials,which improved the loading efficiency and stability of horseradish peroxidase(HRP),and used it for the sensitive colorimetric detection of hypoxanthine(HX)in oysters.As the product of the reaction be-tween HX and xanthine oxidase(XOD),the H2O2 intermediate can oxidize the Amplex red(AR),and form a red product under the catalysis of the Fe3O4@ZIF-8@HRP@SiO2 nanoenzyme complex,thus enabling the quantitative analysis of HX.The material's morphology,elemental composition,and surface functional groups were characterized using transmission electron microscopy(TEM),Fourier transform infrared spectroscopy(FTIR),and particle size analysis.Under the optimal condi-tions,the detection range for HX is 0.005-50 μmol/L,with a limit of detection of 0.001 27 μmol/L.Compared with the traditional methods such as capillary electrophoresis and electrochemical methods,it has a wider linear range and higher detection sensitivity.This method was also used for detecting the HX content changes in oysters that were stored continuously for 96 hours at 4℃and 30℃,fur-ther verifying its feasibility for detecting actual samples.关键词
次黄嘌呤(HX)/辣根过氧化物酶/新鲜度/酶固定化/比色检测Key words
hypoxanthine(HX)/horseradish peroxidase/freshness/enzyme immobilization/col-orimetric determination分类
化学化工引用本文复制引用
姚梦迪,高宇,孙舒扬,林立,张诺宣,王平,王承克..负载HRP的纳米复合酶用于比色法测定牡蛎中的次黄嘌呤[J].分析测试学报,2025,44(10):2191-2197,7.基金项目
2021年山东省高等学校"青创人才引育计划"项目,山东省自然科学基金青年项目(ZR2023MC178) （ZR2023MC178）
