分析测试学报2025，Vol.44Issue(10)：2204-2208,5.DOI:10.12452/j.fxcsxb.25011739
蛋白质凝胶电泳在线分析方法的研究
Research on Online Analysis of Protein by Gel Electrophoresis
摘要
Abstract
Based on the self-built protein electrophoresis instrument,this paper proposes a method that can realize the SDS-PAGE separation of proteins,and online analysis of its molecular weight and concentration.Through electrophoresis of 10.5-107 kDa protein Marker,and the relationship between its gray value and protein molecular concentration is analyzed by analysis of its gel electro-phoresis images.Results showed that:the device can achieve rapid separation of protein samples with a molecular weight of less than 107 kDa within 30 min,and the fluorescence intensity of each protein band reaches its peak when irradiated with ultraviolet light for 5 min;Within the range of 10.5-107 kDa,the migration distance is directly proportional to the molecular weight,with a corre-lation coefficient of up to 0.93;For protein molecules above 20.1 kDa,the fluorescence intensity shows a linear relationship in the concentration range of 0.72-3.96 μg/μL with with correlation coef-ficients more than 0.988;Using the developed protein electrophoresis instrument analyzing the pro-tein components in tears,and immunoglobulin A,lactoferrin,serum protein,lipid transport pro-tein,and lysozyme were successfully separated from tears.Compared with the traditional protein gel electrophoresis methods,the developed self-built protein electrophoresis instrument could shorten the protein electrophoresis time from over 1.5 h to within 30 min.This study was important for the de-velopment of rapid protein molecule detection equipment.关键词
蛋白质/十二烷基硫酸钠-聚丙烯酰胺凝胶电泳/荧光染料/泪液Key words
protein/sodium dodecyl sulfate-polyacrylamide gel electrophoresis/fluorescent dye/tear分类
化学化工引用本文复制引用
樊帅强,游庆祥,曾媛,杨波,马鸣远,张大伟,李振庆..蛋白质凝胶电泳在线分析方法的研究[J].分析测试学报,2025,44(10):2204-2208,5.基金项目
上海市"科技创新行动计划"生物医药科技支撑专项(19ZR1477500) （19ZR1477500）
