分析化学2025，Vol.53Issue(9)：1403-1410,8.DOI:10.19756/j.issn.0253-3820.251172
凝血酶电化学检测方法的研究进展
Progress in Methods for Electrochemical Detection of Thrombin
摘要
Abstract
As a serine protease,thrombin can convert soluble fibrinogen into insoluble fibrin and plays a pivotal role in the coagulation cascade.Therefore,the accurate quantitative assay of thrombin levels is of great value in the evaluation of coagulation function,clinical screening and prognostic monitoring of coagulation-related diseases,and screening of drugs for targeted therapy.Existing methods for thrombin detection can be divided into two categories,e.g.,the assay of concentration levels using nucleic acid aptamers as the affinity elements and the assay of activity levels based on the hydrolytic cleavage of substrate peptides.In recent years,electrochemical biosensors have attracted much attention in thrombin detection due to high sensitivity,high selectivity,simple instrument,fast response,and good portability.In this review,the latest research progress in methods for electrochemical detection of thrombin was summarized,focusing on the detection principles and the applied signal amplification strategies of related electrochemical biosensors.In addition,the challenges with respect to the practical use of electrochemical thrombin biosensors and the prospects were discussed.关键词
凝血酶/电化学生物传感器/核酸适配体/底物肽/评述Key words
Thrombin/Electrochemical biosensor/Nucleic acid aptamer/Substrate peptide/Review引用本文复制引用
吴迪,张茜瑶,许靖靖,陈艺婷,唐文琪,许文慧,陈松民,胡琼,牛利..凝血酶电化学检测方法的研究进展[J].分析化学,2025,53(9):1403-1410,8.基金项目
广东省普通高校特色创新项目(No.2024KTSCX154)和广州大学大学生创新训练项目(Nos.XJ202511078251,S202411078056)资助. Supported by the Project of the Department of Education of Guangdong Province(No.2024KTSCX154)and the Innovation Training Program for College Students of Guangzhou University(Nos.XJ202511078251,S202411078056). （No.2024KTSCX154）
