分析化学2025，Vol.53Issue(9)：1447-1456,10.DOI:10.19756/j.issn.0253-3820.251181
基于环介导等温扩增技术的微流控芯片在食源性致病菌快速检测中的研究进展
Advances in Microfluidic Chip Based on Loop-mediated Isothermal Amplification Technology for Rapid Detection of Foodborne Pathogens
摘要
Abstract
Loop-mediated isothermal amplification(LAMP)is a newin vitronucleic acid amplification technique,which has been widely used in rapid detection of pathogenic bacteria,with advantages of high efficiency,simple operation and low cost.Microfluidic chip technique is a kind of miniaturized and integrated analytical technology,which integrates automation and high throughput,and can effectively avoid sample cross-contamination and has the advantages of high sample utilization rate and high cost-effectivenes.LAMP combined with microfluidic chip can detect multiple samples of the same pathogen at the same time or single samples of different pathogens at the same time,providing a new method for the rapid,field detection of pathogens.In this paper,the research progresses of LAMP microfluidic chip and its application in rapid detection of pathogenic bacteria in recent years were reviewed,and the future prospect was discussed.关键词
环介导等温扩增/微流控芯片/食源性致病菌/快速检测/评述Key words
Loop-mediated isothermal amplification/Microfluidic chip/Foodborne pathogens/Rapid detection/Review引用本文复制引用
潘燕竹,唐家华,柴跃香,束璐茜,谢佳欣,张炎..基于环介导等温扩增技术的微流控芯片在食源性致病菌快速检测中的研究进展[J].分析化学,2025,53(9):1447-1456,10.基金项目
国家自然科学基金项目(No.32460624)、昆明理工大学"双一流"创建联合项目(No.202201BE070001-028)、云南省教育科学规划项目(No.BC23002)、第十九届"挑战杯"全国大学生课外学术科技作品竞赛培育项目暨2024年学生课外学术科技创新基金项目(No.2024ZK128)资助. Supported by the National Natural Science Foundation of China(No.32460624),the Kunming University of Science and Technology″Double First-class″Creation Joint Project(No.202201BE070001-028),the Educational Science Planning Project of Yunnan Province(No.BC23002),the Cultivation Project of the 19th ″Challenge Cup″ National Extracurricular Academic and Scientific Works Competition for College Students of Kunming University of Science and Technology and the Student Extracurricular Academic and Scientific Innovation Fund Project in 2024(No.2024ZK128). （No.32460624）
