分析化学2025，Vol.53Issue(9)：1457-1466,10.DOI:10.19756/j.issn.0253-3820.251155
基于AuNPs-FeCDs双纳米酶级联体系结合智能手机的葡萄糖高灵敏检测方法
AuNPs-FeCDs Dual Nanozyme Cascade System Integrated with A Smartphone Platform for Sensitive Detection of Glucose
摘要
Abstract
A centrifugation-free,single-reaction colorimetric method for detection of glucose,utilizing a dual nanozyme cascade system based on gold nanoparticles(AuNPs)and iron-doped carbon dots(FeCDs),was developed in this work.The AuNPs exhibited glucose oxidase-like activity to catalyze glucose oxidation for generation of H2O2,while the FeCDs demonstrated peroxidase-like activity to subsequently catalyze the H2O2-mediated oxidation of 3,3',5,5'-tetramethylbenzidine(TMB).To prevent interference from the blue signal generated by self-aggregation of AuNPs in subsequent quantitative detection,the reaction system was terminated with HCl,converting oxTMB into a stable yellow product.Based on changes in the absorbance at 450 nm of this yellow solution,a quantitative relationship was established between glucose concentration and absorbance at 450 nm(A450).Experimental results demonstrated that this sensor achieved a linear detection range of 44 μmol/L to 11.11 mmol/L(R2=0.993)with a detection limit of 30.68 μmol/L and spiked recoveries of 97.9%-104.7%.By integrating smartphone-based color recognition capabilities,a rapid visual detection platform was established for quantification of glucose through RGB analysis.The validation experimental results using commercial glucose injection samples further confirmed the practical application potential of this methodology.关键词
金纳米颗粒/碳点/纳米酶/级联体系/葡萄糖检测Key words
Gold nanoparticles/Carbon dots/Nanozymes/Cascade system/Glucose detection引用本文复制引用
叶清菁,周雪英,郑颜盈,张云,金文英,袁亚利..基于AuNPs-FeCDs双纳米酶级联体系结合智能手机的葡萄糖高灵敏检测方法[J].分析化学,2025,53(9):1457-1466,10.基金项目
国家自然科学基金项目(No.22364011)和广西自然科学基金项目(No.2025GXNSFAA069229)资助. Supported by the National Natural Science Foundation of China(No.22364011)and the Guangxi Natural Science Foundation(No.2025GXNSFAA069229). （No.22364011）
