分析化学2025，Vol.53Issue(9)：1467-1475,9.DOI:10.19756/j.issn.0253-3820.251156
基于碳点-介孔二氧化硅纳米荧光探针的高灵敏侧流免疫分析平台检测白细胞介素-6
A Sensitive Lateral Flow Immunoassay for Detection of Interleukin-6 Using Carbon Dots-Mesoporous Silica Nanocomposite Fluorescent Probes
摘要
Abstract
In this study,a sensitive lateral flow immunoassay(LFIA)platform based on carbon dots-mesoporous silica nanocomposite(CD-MSNs)fluorescent probes was constructed for high-performance detection of inflammatory marker interleukin-6(IL-6).Green fluorescent carbon dots(CDs)were prepared by hydrothermal method with 3,9-perylenic acid and 3-aminopropyltriethoxysilane(APTES)as raw materials,and highly fluorescent CD-MSNs composites were then constructed by encapsulating the prepared CDs in mesoporous silica nanoparticles(MSNs).Fluorescent probes were prepared by covalent coupling of CD-MSNs with IL-6 antibody.Fluorescent immunochromatographic test strips were constructed by spraying IL-6 capture antibody and goat anti-mouse IgG on nitrocellulose membrane as detection line(T-line)and quality control line(C-line),respectively.The fluorescence immunoassay analyzer was used to quantitatively detect the fluorescence intensity of T-line,and the experimental results showed that the LFIA platform based on this probe had a good linear relationship in IL-6 concentration range of 102-106 pg/mL,and the detection limit was 64 pg/mL,which was two orders of magnitude more sensitive than that of the traditional colloidal gold test strips.This method effectively solved the issue of insufficient sensitivity of traditional LFIA technique,and provided a rapid and highly sensitive detection method for early diagnosis of inflammatory diseases.关键词
碳点/介孔二氧化硅/侧流免疫分析/白细胞介素-6Key words
Carbon dots/Mesoporous silica/Lateral flow immunoassay/Interleukin-6引用本文复制引用
杨月倩,王鹏跃,任嘉琪,潘宵,谭峰华,马玉杰,温聪颖,曾景斌..基于碳点-介孔二氧化硅纳米荧光探针的高灵敏侧流免疫分析平台检测白细胞介素-6[J].分析化学,2025,53(9):1467-1475,9.基金项目
国家自然科学基金项目(No.22476214)、山东省自然科学基金项目(No.ZR2022JQ07)、泰山学者青年专家项目(No.tsqn202211080)、中央高校基本科研业务费项目(No.24CX03011A)和青岛市自然科学基金重点项目(No.24-8-4-zrjj-9-jch)资助. Supported by the National Natural Science Foundation of China(No.22476214),the Key Fundamental Project of Shandong Natural Science Foundation(No.ZR2022JQ07),the Taishan Scholarship of Shandong Province(No.tsqn202211080),the Fundamental Research Funds for the Central Universities(No.24CX03011A)and the Key Fundamental Project of Qingdao City(No.24-8-4-zrjj-9-jch). （No.22476214）
