分析化学2025，Vol.53Issue(9)：1476-1485,10.DOI:10.19756/j.issn.0253-3820.241439
基于氯化血红素-还原氧化石墨烯-二氧化锰纳米酶的比色传感器检测高尔基体蛋白73
Colorimetric Sensor for Determination of Golgi Protein 73 Based on Hemin-Reduced Graphene Oxide-Manganese Dioxide Nanozyme
摘要
Abstract
Hepatocellular carcinoma(HCC)is one of common cancer that seriously endangers human health.Designing methods for early,rapid,and accurate diagnosis of HCC has become the key point.Golgi protein 73(GP73),a novel potential biomarker for HCC,is crucial for diagnosis and treatment of HCC.In this study,a colorimetric sensor with rapidity,smplicity and high specificity was established for detection of GP73 based on peroxidase-like activity of hemin-reduced graphene oxide-manganese dioxide(H-rGO-MnO2).The H-rGO-MnO2-GP73Apt1 signal probe was synthesized by carboxyl of H-rGO-MnO2 nanozyme and amination of GP73 aptamer(GP73Apt1)though amide reaction.In the presence of GP73,the sulfhydryl-modifed GP73 aptamer(GP73Apt2),as the capture probe,and the signal probe both specifically recognized GP73,forming a sandwich structure(GP73Apt2-GP73-H-rGO-MnO2-GP73Apt1).This structure could catalyze the oxidation of H2O2 to produce hydroxyl radical(·OH),thereby oxidizing the colorless phthalenediamine(OPD)into the yellow 2,3-diaminophenazine(DPA).The quantitative detection of GP73 was achieved by measuring the characteristic absorbance of DPA at 450 nm.In the GP73 concentration range of 10-150 ng/mL,there was a good linear relationship between the DPA absorbance at 450 nm(A450 nm)and the GP73 concentration under optimal conditions.The linear equation was A450 nm=0.00321CGP73+0.8988,with the correlation coefficient(R2)of 0.9960 and the detection limit(LOD)of 5.38 ng/mL.The colorimetric sensor was applied to detection of GP73 in human serum samples,with recoveries of 88.4%‒98.8%.This sensor showed high specificity,sensitivity,and stability,and had potential for clinical detection of GP73,providing a new approach for the early diagnosis of HCC.关键词
纳米酶/比色传感器/肝细胞癌/高尔基体蛋白73/类过氧化物酶Key words
Nanozyme/Colorimetric sensor/Hepatocellular carcinoma/Golgi protein 73/Peroxidase-like activity引用本文复制引用
谭晓红,周嘉浩,许沛鸿,林浩,李桂银..基于氯化血红素-还原氧化石墨烯-二氧化锰纳米酶的比色传感器检测高尔基体蛋白73[J].分析化学,2025,53(9):1476-1485,10.基金项目
大学生创新创业训练计划项目(No.71013407217)、"攀登计划"(2020-2024年)项目(No.pdjh202360359)和广东石油化工学院高层次人才引进项目(No.RC-XJ2022000401)资助. Supported by the Projects of College Students'Innovation and Entrepreneurship Training(No.71013407217),the Projects of″Climbing Plan″(2020-2024)(No.pdjh202360359)and the Projects of Talents Recruitment of GDUPT(No.RC-XJ2022000401). （No.71013407217）
