分析化学2025，Vol.53Issue(9)：1486-1495,10.DOI:10.19756/j.issn.0253-3820.251136
一锅法合成巯基保护的荧光银纳米簇用于检测四环素
One-pot Synthesis of Sulfhydryl-protected Fluorescent Silver Nanoclusters and Its Application in Detection of Tetracycline
摘要
Abstract
Water-soluble silver nanoclusters(DHLA-AgNCs)with red fluorescence emission were synthesized using silver nitrate(AgNO3)as silver source,dihydrolipoic acid(DHLA)as ligand and sodium borohydride(NaBH4)as reducing agent by one-pot method.Based on the selective quenching of DHLA-AgNCs by tetracycline(TC),a rapid and selective fluorescence analysis method for detection of TC was constructed by monitoring the fluorescence intensity change at 650 nm.Under the optimal detection conditions,the fluorescence quenching efficiency of DHLA-AgNCs showed good linear relationship with concentration of TC within the range of 10.0-120.0 μmol/L,and the limit of detection(LOD)was 0.39 μmol/L.This method was successfully applied to detection of TC in milk samples,with spiked recoveries ranging from 99.5%to 102.5%,and relative standard deviations(RSDs)less than 5%.This method had the advantages of simplicity,rapidity,strong specificity and low cost,and thus provided a simple and feasible strategy for selective detection of TC.关键词
银纳米簇/四环素/荧光检测/纳米材料Key words
Silver nanoclusters/Tetracycline/Fluorescence detection/Nanomaterials引用本文复制引用
杨喜樑,况亚亚,李梓韬,曹秋娥,周川华..一锅法合成巯基保护的荧光银纳米簇用于检测四环素[J].分析化学,2025,53(9):1486-1495,10.基金项目
大学生创新创业训练项目(No.202410673105)资助. Supported by the National Undergraduate Training Program for Innovation and Entrepreneurship(No.202410673105). （No.202410673105）
