分析化学2025，Vol.53Issue(9)：1496-1504,9.DOI:10.19756/j.issn.0253-3820.251031
基于H+诱导i-Motif的荧光传感器在微藻固定CO2技术中的应用
Application of Fluorescence Sensor Based on H+-Induced i-Motif in Microalgae CO2 Fixation Technology
摘要
Abstract
Microalgae can achieve sustainable CO2 fixation and biomass conversion,making them a green path for carbon capture and conversion.Monitoring extracellular carbonic anhydrase(CA)activity in microalgae is crucial for regulating carbon conversion efficiency.In this study,double-stranded DNA was designed as a capture probe to bind H+,which was produced by CA-catalyzed CO2 hydration.This interaction induced conformational changes in the DNA probe,enhancing fluorescence signals and thereby establishing a recognition and signal transduction mechanism for CA catalytic product.This approach enabled a novel method for measuring CA activity.The response of probe to H+was investigated using ultraviolet visible(UV-Vis)spectroscopy,circular dichroism sepctroscopy,and fluorescence spectroscopy.The experimental results indicated that the fluorescence intensity of the probe increased progressively with rising H+concentration.Using this probe,CA activity detection could be completed within 29 min,with a detection range of 0.35-17500 U/mL and a detection limit of 0.15 U/mL.Compared to traditional methods,this approach offered a lower detection limit and was suitable for analyzing and detecting extracellular CA activity in actual algal samples.关键词
微藻碳捕获和转化/碳酸酐酶/荧光传感器/DNA探针/i-Motif结构Key words
Microalgae carbon capture and conversion/Carbonic anhydrase/Fluorescent sensor/DNA probe/i-Motif structure引用本文复制引用
杨哲涵,王雨涵,杨镇嘉,殷杰..基于H+诱导i-Motif的荧光传感器在微藻固定CO2技术中的应用[J].分析化学,2025,53(9):1496-1504,9.基金项目
国家自然科学基金项目(No.21804014)、重庆市自然科学基金项目(No.2023jcyjA3529)、重庆市教委科技项目(No.KJQN202200832)和重庆市教委研究生联合基地培养项目(No.yjd223005)资助. Supported by the National Natural Science Foundation of China(No.21804014),the Natural Science Foundation of Chongqing Science&Technology Commission(No.2023jcyjA3529),the Science and Technology Research Program of Chongqing Municipal Education Commission(No.KJQN202200832)and the Construction of Graduate Joint Training Base of Chongqing Municipal Education Commission(No.yjd223005). （No.21804014）
