分析化学2025，Vol.53Issue(9)：1505-1515,中插4-中插5,13.DOI:10.19756/j.issn.0253-3820.251140
婴幼儿配方乳粉中多组分脂肪酸校准方法对测量的影响
Impact of Multi-Component Fatty Acid Calibration Methods on Quantification of Infant Formula
摘要
Abstract
Infant formula milk powder(infant formula)contains a variety of fatty acids that require hydrolysis and derivation,and the calibration methods are complex and variable,affecting the accuracy of quantification.Using the gas chromatography with flame ionization detector(GC-FID)under internal standard and external standard calibration methods,the effects of using fatty acid methyl esters(FAMEs)and triacylglycerol(TAGs)as calibration solutions on determination of 19 kinds of fatty acids content in infant formula were compared in this work.Additionally,the differences in determination between the acetyl chloride methanol method and the hydrolysis extraction method using FAMEs as the calibration solution under internal standard method were also compared.The quantitative results of TAGs external standard quantitative method were significantly higher than those of FAMEs external standard quantitative method and FAMEs calibrator derived by methylation,and the deviation of quantitative results were 7%-20%and 2%-10%,respectively.The quantitative results of FAMEs calibrator added FAME internal standard were significantly higher than those of FAMEs calibrator added FAME internal standard derived from TAG methylation and FAMEs calibrator added TAG internal standard with methyl esterification,and the deviation of quantitative results were about 15%and 2%-5%,respectively.The results indicated that both the two methods of internal standard calibration using FAMEs as the calibration solution and external standard calibration using TAGs as the calibration solution could effectively eliminate the bias in the determination,with simple operation and accurate comparation of the results.However,the results were significantly lower under the external standard calibration using FAMEs as calibration solution.关键词
脂肪酸/婴幼儿配方乳粉/定量校准方法/气相色谱氢火焰测定法Key words
Fatty acid/Infant formula/Quantitative calibration method/Gas chromatography flame ionization detector引用本文复制引用
陈晓婷,徐梦倩,张庆合,李秀琴..婴幼儿配方乳粉中多组分脂肪酸校准方法对测量的影响[J].分析化学,2025,53(9):1505-1515,中插4-中插5,13.基金项目
国家重点研发计划项目(No.2022YFF0710400)资助. Supported by the National Key Research and Development Program of China(No.2022YFF0710400). （No.2022YFF0710400）
