高师理科学刊2025，Vol.45Issue(9)：56-62,79,8.DOI:10.3969/j.issn.1007-9831.2025.09.009
碳酸盐黑钙土细菌群落及酶活性对玉米-大豆轮作的响应
Response of bacterial community and enzyme activity in carbonate chernozem soil to maize-soybean rotation
摘要
Abstract
Continuous cropping obstacles refer to the phenomenon of soil microecological imbalance and productivity decline caused by long-term consecutive cultivation of the same crop in a single field.To investigate the mechanisms by which maize-soybean rotation alleviates continuous cropping obstacles through regulating soil enzyme activities and rhizobacterial community structure in carbonate chernozem soils of the semi-arid western Heilongjiang Province,established four treatments:soybean-maize-soybean rotation(RS),maize-soybean-maize rotation(RM),continuous soybean(CS)and continuous maize(CM).By combining Illumina high-throughput sequencing to analyze the structure and composition of bacterial communities in the crop rhizosphere soil,soil enzyme activities were also measured.The results demonstrated that rotation significantly enhanced soil sucrase,catalase,urease and alkaline phosphatase activities.Bacterial community similarity between RS and RM treatments was higher than that in continuous cropping,with crop species exerting a stronger influence on bacterial communities than planting patterns.Acidobacteria and Chloroflexi remained dominant bacterial phyla across all treatments,while rotation increased the relative abundance of Proteobacteria.Redundancy analysis identified available phosphorus,available potassium,total phosphorus and total potassium as key factors regulating bacterial communities,showing significant correlations with 12 bacterial orders,including Rhizobiales.The study confirms that crop rotation mitigates continuous cropping obstacles by enhancing soil enzyme activities,optimizing nutrient cycling and reconstructing rhizosphere microecology.关键词
玉米-大豆轮作/连作障碍/土壤酶/根际细菌群落Key words
maize-soybean rotation/continuous cropping obstacles/soil enzymes/rhizosphere bacterial community分类
生物科学引用本文复制引用
斗燕慧,康霄,王名扬,吴天昊,黄荣雁,李馨园..碳酸盐黑钙土细菌群落及酶活性对玉米-大豆轮作的响应[J].高师理科学刊,2025,45(9):56-62,79,8.基金项目
高教强省专项资金大学生创新创业项目(150111124101) （150111124101）
黑龙江省省属高校基本科研业务费(145309629,135409419) （145309629,135409419）
