东南园艺2025，Vol.13Issue(3)：241-247,7.DOI:10.20023/j.cnki.2095-5774.2025.03.002
不同开花期含笑花被片挥发性成分的HS-SPME/GC-MS分析
Analysis of volatile components of Michelia figo tepals at different flowering stages by HS-SPME/GC-MS
摘要
Abstract
[Objective]In order to further investigate the aroma composition and its impact factors of the Michelia figo tepals at different flowering stages.[Method]The volatile components of the tepals of M.figo at initial flowering stage,full flowering stage,and decay stage were determined by headspace solid-phase microextraction(HS-SPME)combined with gas chromatography-mass spectrometry(GC-MS).[Result]In this study,a total of 55 volatile organic compounds were detected,including 29 terpenes,16 esters,2 acids,3 alcohols,4 aldehydes and 1 ketone.Among them,45,36,and 25 volatiles were identified at the initial flowering stage,full flowering stage,and decay stage,respectively.Among these volatile organic compounds,19 components were detected at different flowering stages.In particular,β-elemene had the highest content,reaching 60.49,35.52 and 24.69 μg/kg at the initial flowering stage,full flowering stage,and decay stage,respectively.Volatiles such as isobutyl acetate,γ-elemene,β-copaene,ethyl-2-methylpropanoate,ethyl hexanoate,and isobutyl hexanoate also had relatively high contents at these three stages.Odor activity value(OAV)analysis showed that there were six characteristic aroma components in M.figo tepals.Among these characteristic aroma components,β-elemene was common at all three stages,ethyl hexanoate and isobutyl acetate were shared by the initial and full flowering stage,ethyl acetate was unique at the initial flowering stage,γ-selinene and ethyl 2-methylbutyrate were unique to the full flowering stage.[Conclusion]There were significant differences in type number,content and OAV value in M.figo tepals at different flowering stages.Differences in the content of compounds such as β-elemene,ethyl hexanoate,isobutyl acetate,ethyl acetate,γ-selinene and ethyl 2-methylbutyrate were the main reasons for the difference in the floral aroma across different stages.关键词
含笑/顶空固相微萃取/气相色谱—质谱联用/挥发性有机化合物/香气特征Key words
Michelia figo/HS-SPME/GC-MS/volatile organic compounds/aroma characteristic分类
农业科技引用本文复制引用
李隆达,沈燊,赵俊,曹小倩,李国鹏..不同开花期含笑花被片挥发性成分的HS-SPME/GC-MS分析[J].东南园艺,2025,13(3):241-247,7.基金项目
广东海洋大学大学生创新创业项目(S202210566034) （S202210566034）
