广西植物2025，Vol.45Issue(9)：1607-1616,10.DOI:10.11931/guihaia.gxzw202404020
广西红树林放线菌Streptomyces sporoverrucosus 33510次级代谢产物研究
Secondary metabolite study from Guangxi mangrove-derived actinomyces Streptomyces sporoverrucosus 33510
摘要
Abstract
In order to study the secondary metabolites and antifungal activities of mangrove-derived actinomyces Streptomyces sporoverrucosus 33510 in Guangxi.Silica gel column chromatography,ODS reversed phase column chromatography,and hemi-preparative high performance liquid chromatography combined with various chromatographic techniques were used to separate and purify the fermentation extract of actinomyces No.2 medium of this strain.The structures of monomeric compounds were identified by spectral analysis and literature comparison.In addition,the antifungal activity of these monomeric compounds were tested by disk diffusion assay.The results were as follows:(1)Ten monomeric compounds were isolated from fermentation cultures of Streptomyces sporoverrucosus 33510 and identified as cyclo(L-isoleucyl-L-prolyl-L-leucyl-L-prolyl)(1),matsudipeptide B(2),cyclo(L-Leu-L-Pro)(3),cyclo(Leu-Ile)(4),N-acetyltyramine(5),isoleucine(6),p-hydroxybenzoic acid(7),3-hydroxy-4-methoxybenzoic acid(8),2-minaline(9),2-hydroxybenzaldehyde(10).Compounds 1,2,4,5,7,8,and 10 were all isolated for the first time from this strain.(2)The activity test results indicated that Compound 6 exhibited high sensitivity against the Cryphonectria parasitica at a concentration of 0.2 mmol·L-1,and the effect was superior to that of the positive control group.In this paper,the activity of Compound 6 against C.parasitica was reported for the first time.This study further enriches the structural types of secondary metabolites produced by the mangrove-derived actinomyces Streptomyces sporoverrucosus 33510 in Guangxi.The antifungal components of strain 33510 are identified,providing a theoretical basis for the subsequent development and utilization of antifungal pesticides.关键词
Streptomyces sporoverrucosus/次级代谢产物/分离纯化/结构鉴定/抑菌活性Key words
Streptomyces sporoverrucosus/secondary metabolites/separation and purification/structure identification/antifungal activity分类
农业科技引用本文复制引用
翁杰,刘颖,章唐桀,杨淑玉,吴金城,刘洪存,孟娟,姜明国,杨立芳..广西红树林放线菌Streptomyces sporoverrucosus 33510次级代谢产物研究[J].广西植物,2025,45(9):1607-1616,10.基金项目
国家自然科学基金(81960164) （81960164）
广西重点研发计划项目(桂科AB21196020) （桂科AB21196020）
2023年度广西少数民族预科教育基地科研项目(YJJDA202301). （YJJDA202301）
