广西植物2025，Vol.45Issue(9)：1617-1627,11.DOI:10.11931/guihaia.gxzw202502017
北部湾红树土壤真菌-细菌共生体多样性及与野生线虫生态关系研究
Diversity of fungal-bacterial symbionts in the Beibu Gulf mangrove soil and their ecological relationships with wild nematodes
摘要
Abstract
To study the diversity of fungal-bacterial symbionts in the Beibu Gulf mangrove soil habitats and their ecological relationships with wild nematodes,the microbial interactions within mangrove soil habitats through a multi-methodological approach were systematically investigated.In view of this,mangrove soil samples were collected from the Beibu Gulf region,and pure culture methods were employed to isolate and purify fungal strains.The 16S rRNA primer was used to screen fungal-bacterial symbionts and analyze the diversity of endosymbiotic bacteria.The ITS primer was used to analyze fungal host diversity.The wild nematodes were isolated and identified from mangrove soil.And 20%fermentation broth was used to explore the relationship between symbionts and wild nematodes.The results were as follows:(1)A total of 50 strains of fungi were isolated from 14 mangrove soil samples,and 29 pairs fungal-bacterial symbionts were obtained.The endosymbiotic bacteria included the genera Burkholderia,Pantoea,Sphingomonas and Providencia,among which 20 strains belonged to the genus Burkholderia,which widely formed symbiosis with fungal hosts.According to the identification of ITS,29 pairs of symbiont host fungi belonged to 2 phyla,4 classes,7 orders,11 families,12 genera and 22 species.(2)The endosymbiont bacteria were isolated from the symbiont GXIMD14377 derived from the soil of Hibiscus tiliaceus in Qinzhou via the hyphal fragmentation method and identified as Pantoea dispersa through 16S rRNA gene sequencing.(3)The wild nematodes fed on P.dispersa were isolated and cultured from the soil of Hibiscus tiliaceus in Qinzhou,and were identified as Caenorhabditis elegans.(4)It was found that fermentation broths of some symbionts exhibited antagonistic effects on wild nematodes.Among them,the symbiont GXIMD14385(Fusarium solani-Burkholderia territorii)showed the strongest antagonistic effect on wild nematodes,with an corrected mortality of(84.25±3.61)%.Furthermore,the symbiont GXIMD14377(Fusarium oxysporum-Pantoea dispersa)showed antagonistic effects on wild nematodes,which had predation relationships with symbiotic bacteria.In conclusion,the results indicate that the mangroves in the Beibu Gulf have a rich diversity of fungal-bacterial symbionts.There is a tripartite relationship exists between symbionts,endosymbiotic bacteria,and wild nematodes in the soil habitat of mangroves.This study is the first to explore the ecological relationship between fungal-bacterial symbionts and wild nematodes in mangrove habitats.This study lays the foundation for investigating the fungal-bacterial symbiotic interactions and their ecological relationships with wild nematodes in mangrove soils.关键词
红树林/红树土壤/真菌-细菌共生体/共生体多样性/真菌内生细菌/土壤线虫Key words
mangroves/mangrove soils/fungal-bacterial symbionts/symbiont diversity/fungal endophytic bacteria/soil nematodes分类
农业科技引用本文复制引用
徐淑芬,李蜜,陈心仪,许岚笛,易湘茜,高程海..北部湾红树土壤真菌-细菌共生体多样性及与野生线虫生态关系研究[J].广西植物,2025,45(9):1617-1627,11.基金项目
广西重点研发计划项目(AB24010109) （AB24010109）
国家自然科学基金(42466005) （42466005）
广西青年岐黄学者培养项目(GXQH202412) （GXQH202412）
广西研究生教育创新计划项目(YCSW2024421) （YCSW2024421）
广西中医药大学岐黄工程高层次人才团队培育项目(202404) （202404）
广西中医药大学高层次人才培育创新团队项目(2022A007) （2022A007）
广西中医药大学桂派杏林拔尖人才资助项目(2022C008) （2022C008）
广西中医药大学研究生教育创新计划项目(YCSY2023066). （YCSY2023066）
