广西植物2025，Vol.45Issue(9)：1641-1653,13.DOI:10.11931/guihaia.gxzw202410040
橡胶树葡萄糖转运蛋白(HbGLUTs)基因家族的鉴定与分析
Identification and analysis of glucose transporter proteins(HbGLUTs)gene family in rubber tree
摘要
Abstract
Glucose is not only the basic energy source for organisms but also serves as a precursor in various synthetic metabolic processes.Glucose transport proteins(GLUTs)can regulate glucose levels within organisms and play a crucial role in maintaining glucose homeostasis.Currently,the functions of the GLUT family in rubber trees(Hevea brasiliensis)and their roles in key biological processes such as sugar homeostasis and latex regeneration remain unclear.Therefore,this study employed bioinformatics methods to identify GLUTs in rubber trees and systematically analyzed the physicochemical properties,protein structures,conserved motifs,phylogenetic development,cis-acting element structural features,and gene expression patterns of the gene family members.The results were as follows:(1)A total of 31 members of the HbGLUTs family members(HbGLUT1-HbGLUT31)were identified,all located in the cell membrane,with the number of transmembrane domains ranging from 9 to 24(both HbGLUT1 and HbGLUT2 contained 24 transmembrane domains),and the secondary structure was primarily composed of α-helix.(2)Evolutionary analysis revealed that HbGLUTs were functionally similar to human GLUTs in terms of classification.Type I subfamily members(such as HbGLUT8 and HbGLUT15)had conserved hexose transport functions and maintained latex production homeostasis by rapidly supplementing glucose during latex regeneration;it is speculated that TypeⅡand Type Ⅲsubfamily members evolved diverse transport capabilities for substrates such as fructose and myo-inositol.Among them,TypeⅢsubfamily members(such as HbGLUT3,HbGLUT17,HbGLUT18)were highly expressed in various tissues,and their promoters contained stress-related elements,indicating their involvement in plant stress responses.(3)Tissue-specific expression analysis showed that HbGLUTs were generally expressed at low levels in latex,but some genes of Type I subfamily were crucial for latex regeneration.Additionally,several members(such as HbGLUT21 and HbGLUT28,etc.)were highly expressed specifically in flowers,supporting the high energy requirements of the reproductive process.This study provides important clues for elucidating the functions of HbGLUTs in latex regeneration and stress responses,laying a foundation for further research on sugar transport proteins in rubber trees.关键词
橡胶树/HbGLUTs/结构特征/理化特性/表达模式Key words
rubber tree(Hevea brasiliensis)/HbGLUTs/structural features/physicochemical properties/expression pattern分类
农业科技引用本文复制引用
薛永康,曾力旺,郝园园,龙翔宇..橡胶树葡萄糖转运蛋白(HbGLUTs)基因家族的鉴定与分析[J].广西植物,2025,45(9):1641-1653,13.基金项目
海南省自然科学基金(323QN298) （323QN298）
海南省重点研发项目(ZDYF2024XDNY234) （ZDYF2024XDNY234）
海南省自然科学基金创新团队项目(322CXTD526). （322CXTD526）
