广西植物2025，Vol.45Issue(9)：1668-1678,11.DOI:10.11931/guihaia.gxzw202408031
沙蒿PP2C2基因的序列分析及其在干旱胁迫下的表达模式
Sequence analysis of PP2C2 gene in Artemisia desertorum and its expression pattern under drought stress
摘要
Abstract
Protein phosphatase 2C(PP2C)play important roles in growth and development,cell cycle regulation,signal transduction,and responses to environmental stress.This study focused on Artemisia desertorum,a highly drought-tolerant and multifunctional plant native to arid desert regions,to investigate the functional role of PP2C2 gene in drought tolerance.Using A.desertorum as the experimental material,the PP2C2 gene was amplified,its sequence was subjected to bioinformatic analysis,and its protein structure was predicted.Additionally,the expression patterns of PP2C2 under various water stress conditions were analyzed,and the regulatory role of the PP2C2 gene in drought stress responses was elucidated.The results were as follows:(1)The PP2C2 gene in A.desertorum contained an open reading frame(ORF)of 1404 bp,encoding a protein of 467 amino acids.Sequence alignment demonstrated that the gene shared high homology with PP2C genes from other plants,such as Erigeron canadensis.(2)Bioinformatic analysis revealed that the PP2C2 protein was localized in the nucleus,no signal peptide,and was classified as a non-secretory protein.Its secondary structure predominantly consisted of random coils,with strong hydrophilicity,no transmembrane helical region,and multiple potential modification sites.(3)Quantitative real-time PCR(qRT-PCR)analysis showed that the expression of the PP2C2 gene was responsive to varying levels of drought stress.Under drought conditions,its expression exhibited an overall up-regulation trend,while rehydration led to a significant reduction in expression levels.This study preliminarily reveals that the PP2C2 gene plays a significant role in the drought tolerance mechanism of Artemisia desertorum,offering a theoretical reference for further understanding of its drought resistance mechanism and potential application in genetic engineering.关键词
沙蒿/PP2C2基因/序列分析/蛋白结构预测/基因表达Key words
Artemisia desertorum/PP2C2 gene/sequence analysis/protein structure prediction/gene expression分类
农业科技引用本文复制引用
刘若琪,郭思雨,罗曼,马玉花..沙蒿PP2C2基因的序列分析及其在干旱胁迫下的表达模式[J].广西植物,2025,45(9):1668-1678,11.基金项目
青海省科技厅基础研究计划项目(2023-ZJ-747) （2023-ZJ-747）
青海省"昆仑英才·高端创新创业人才"计划项目(2022). （2022）
