福建农业学报2025，Vol.40Issue(6)：568-576,9.DOI:10.19303/j.issn.1008-0384.2025.06.004
急性低氧胁迫对香鱼鳃和肝脏组织结构及抗氧化指标的影响
Histology and Antioxidant Activity of Gills and Liver of Plecoglossus altivelis under Acute Hypoxic Stress
摘要
Abstract
[Objective]Physiological and biochemical responses of the gills and liver of Plecoglossus altivelis under acute hypoxic exposure were studied.[Methods]Physiological responses of P.altivelis with or without imposed acute hypoxic stress for 6,24,and 48 h in a tank were monitored.A 6 h reoxygenation period was given for the fish prior to analyses on the structural changes as well as antioxidant and metabolic enzyme activities in the gills and liver.[Results]The physiological observation showed in P.altivelis,higher than in benthic fish,a respiration points of head-up at 2.85 mg·L‒1 and asphyxiation at 1.37 mg·L‒1 indicating its high sensitivity to hypoxia.Histologically,the hypoxic stress caused gill lamellae bending and swelling,blood cell accumulation,liver vacuolization,hepatic sinusoids dilatation,and cell necrosis in the fish.Prolonged stress significantly elevated the activities of catalase(CAT),and glutathione peroxidase(GSH-Px)in the gills and liver(P＜0.05)to peak in 24 h and declined to some degree in 48 h.The GSH-Px activity in the gills and liver,meanwhile,became significantly higher than control(P＜0.05)and maximized in 24 h followed by a decrease up to 48 h.The pyruvate kinase(PK)and lactate dehydrogenase(LDH)activities as well as the malondialdehyde(MDA)content rose significantly higher than control(P＜0.05)to top in 48 h.After 6 h of reoxygenation,the activities of the abovementioned 4 enzymes and MDA content decreased but still higher than those of control.More important,the damage incurred to the gills and liver did not fully recover after the reoxygenation.[Conclusion]Although P.altivelis could resist acute hypoxia by physiologically increasing its antioxidant enzyme activities and anaerobic metabolism,prolonged exposure under the stress would cause irreversible histological and enzymatic alternations in the liver and gills once the threshold of tolerance was exceeded.关键词
香鱼/低氧胁迫/鳃/肝脏/生理生化Key words
Plecoglossus altivelis/hypoxic stress/gills/liver/physiology and biochemistry分类
农业科技引用本文复制引用
刘珏巧,朱浩,王飞雨,肖伟,田永华,刘哲,连总强,刘巍,张锋,刘彦斌,刘凯,景梦雷,王永杰,李敏敏,赛清云..急性低氧胁迫对香鱼鳃和肝脏组织结构及抗氧化指标的影响[J].福建农业学报,2025,40(6):568-576,9.基金项目
银川市"三鱼一虾一蟹"科研技术攻关项目(2024年) （2024年）
