福建农业学报2025，Vol.40Issue(6)：585-593,9.DOI:10.19303/j.issn.1008-0384.2025.06.006
月见草全长转录组测序及γ-亚麻酸代谢相关基因鉴定
Full-length Transcriptome and GLA Metabolism-related Genes of Oenothera biennis
摘要
Abstract
[Objective]Full-length transcriptome of Oenothera biennis was sequenced with the genes related to the pharmaceutically interesting metabolites contained in the plant identified.[Method]Roots,stems,leaves,flowers,and seeds of O.biennis were collected for full-length transcriptome sequencing by SMRT for a bioinformatics analysis.Genes associated with γ-linolenic acid metabolism were subsequently identified in the transcriptomic data through homology-based analysis.[Result]There were 843843 circular consensus reads containing 28875 non-redundant unigenes with an average length of 1718 bp and a redundancy-free GC content of 45.6%.The NR database showed the highest similarity of O.biennis with Punica granatum.The GO annotation classified the 18169 unigenes into groups of biological processes,cell components,and molecular functions,while the KEGG did on the 12213 unigenes into 5 major groups with the most,5416 of them,being enriched in metabolic pathways and 6695 in 56 transcription factor families.The 5543 mononucleotide and 5529 trinucleotide repeats were the highest in number.There were 13 pairs of SSR primers in the 6 species of O.bienni that showed polymorphic markers.Twenty of the 50 γ-linolenic acid(GLA)metabolism-related genes identified in the full-length transcripts database were highly expressed in the seeds.[Conclusion]The information obtained would aid gene cloning and functional studies of O.biennis to capitalize the variety of valuable biologically active ingredients such as flavonoids,phenolic compounds,fatty acids,and vitamins contained in the plant.关键词
月见草/全长转录组/测序分析/功能注释/γ-亚麻酸Key words
Oenothera biennis/full-length transcriptome/sequencing/function annotation/γ-linolenic acid分类
农业科技引用本文复制引用
陈岳,佘为为,姚丹青,徐俐琴,徐菲,张微微..月见草全长转录组测序及γ-亚麻酸代谢相关基因鉴定[J].福建农业学报,2025,40(6):585-593,9.基金项目
上海自然科学基金项目(20ZR1439600) （20ZR1439600）
上农植慧种质资源创新团队项目(KY6-0100-24-01) （KY6-0100-24-01）
