福建农业学报2025，Vol.40Issue(6)：610-618,9.DOI:10.19303/j.issn.1008-0384.2025.06.009
糯山药根腐病病原鉴定、培养特性及室内防治药剂筛选
Identification,Culture,and Fungicide Toxicity of Root Rot Pathogens Infecting Glutinous Yams
摘要
Abstract
[Objective]Pathogens that cause root rot on Shaxian glutinous yams were identified,and their culture and effective fungicides were investigated.[Method]Diseased yam samples were collected to isolate and purify the pathogens by tissue separation followed by species identification with Koch's postulates,morphological observation,and multi-locus phylogenetic analysis(ITS-LSU-TUB).Biological characteristics including optimal culture temperature and pH as well as in vitro toxicity of 7 fungicides were determined.[Results]Two strains,SGF03 and SGF08,were isolated and identified as Fusarium solani and Colletotrichum alatae,respectively.The colony diameter of F.solani was significantly larger at 28℃ and pH 7.0,and that of C.alatae,at 28℃ and pH 6.0,than those under other conditions(P<0.05).Among the tested fungicides,AITC,fluazinam,and difenoconazole showed superior efficacy with EC50 of 0.09,1.25,and 6.27 μg·mL-1,respectively,against F.solani and that of 0.08,0.49,and 0.26 μg·mL-1,respectively,against C.alatae.[Conclusion]The Shaxian glutinous yam root rot disease was caused by coinfection of F.solani and C.alatae.In culture,F.solani grew optimally on medium at 28℃ and pH 7.0,and C.alatae at 28℃ and pH 6.0.Fungicides,AITC,fluazinam,and difenoconazole,exhibited significant in vitro effectiveness on the pathogens.关键词
根腐病/镰刀菌/炭疽菌/培养特性/药剂筛选Key words
root rot/Fusarium solani/Colletotrichum alatae/cultural characteristics/fungicide selection分类
农业科技引用本文复制引用
陈姝樽,王荣波,石茗月,刘裴清,李本金,李清,詹梦琳,黄哲鸿,华树妹..糯山药根腐病病原鉴定、培养特性及室内防治药剂筛选[J].福建农业学报,2025,40(6):610-618,9.基金项目
三明市科技计划项目(2024-N-5) （2024-N-5）
