福建农业学报2025，Vol.40Issue(6)：619-627,9.DOI:10.19303/j.issn.1008-0384.2025.06.010
闽北地区牛乳源金黄色葡萄球菌分子流行特征、耐药谱和进化分析
Molecular Epidemiology,Antimicrobial Resistance,and Phylogenetics of Staphylococcus aureus Isolated from Bovine Raw Milk in Northern Fujian,China
摘要
Abstract
[Objective]Molecular epidemiology,antimicrobial resistance,and evolutionary characteristics of Staphylococcus aureus strains isolated from bovine raw milk at dairy farms in northern Fujian,China were investigated.[Methods]From the milk samples collected at 14 dairy farms in 2023-2024,59 pathogen isolates were undergone whole-genome sequencing using the Illumina platform along with molecular MLST and spa typing,antimicrobial susceptibility testing,and resistance gene profiling.Phylogenetic relationship among the isolates was reconstructed based on core genome single-nucleotide polymorphisms(SNPs),and a global minimal spanning tree(MST)analysis conducted using publicly available sequences from the PubMLST database.[Results]Livestock-associated MRSA(LA-MRSA)ST59-t437 was identified for the first time in the dairy specimens from northern Fujian,along with a novel regional clone ST9784-t189.The predominant types of the isolates were ST1-t114(25.4%)and ST9784-t189(20.3%).The overall non-susceptibility rate to antimicrobial agent was 45.8%with the highest resistance to penicillin and ampicillin(28.8%),albeit lower than those found in other regions in China.The spa typing had a high discriminatory power for micro-evolutionary analyses.The MST analysis indicated that ST398 and ST1 had dual-core global dissemination lineages,whereas others had geographically limited distributions.The multidrug-resistant(MDR)strains harbored a rich repertoire of resistance genes showing a significant correlation with the molecular type.[Conclusion]S.aureus from the dairy farms in northern Fujian displayed considerable genetic diversity and relatively low overall antimicrobial resistance.Nevertheless,the newly identified ST9784 and the first regional LA-MRSA ST59-t437 might still pose a risk of dissemination which required attentive cross-host surveillance by the involved agencies.关键词
金黄色葡萄球菌/乳源/分子流行病学/多位点序列分型/抗生素耐药/系统发育分析Key words
Staphylococcus aureus/raw milk/molecular epidemiology/MLST/antimicrobial resistance/phylogenetic analysis分类
医药卫生引用本文复制引用
周双辉,傅德江,陈思琪,陈立文..闽北地区牛乳源金黄色葡萄球菌分子流行特征、耐药谱和进化分析[J].福建农业学报,2025,40(6):619-627,9.基金项目
福建省市场监督管理局科技项目(FJMS2022008) （FJMS2022008）
