福建农业学报2025，Vol.40Issue(6)：628-636,9.DOI:10.19303/j.issn.1008-0384.2025.06.011
姬松茸多糖-铬(Ⅲ)络合物的制备及降血糖活性体外评价
Preparation and In Vitro Hypoglycemic Activity of Chelate Agaricus blazei Polysaccharide-chromium(Ⅲ)Compound
摘要
Abstract
[Objective]Explore the preparation process,structural characteristics,and biological activity of Agaricus blazei polysaccharide-chromium(Ⅲ)complex[ABMP-Cr(Ⅲ)].[Methods]Polysaccharides were extracted from Agaricus blazei by water extraction and alcohol precipitation.The optimum preparation conditions of ABMP-Cr(Ⅲ)were studied by single factor and response surface method of reaction temperature,substrate concentration,reaction time and pH.Its structure was analyzed by scanning electron microscopy and infrared spectroscopy,and its hypoglycemic activity was analyzed by measuring the inhibition rate of α-amylase and α-glucosidase.[Results]The optimum conditions for the preparation of ABMP-Cr(Ⅲ)were as follows:reaction temperature 22.0℃,reaction time 40 min,pH 6.0,substrate concentration 24.00 mg·mL-1.Under these conditions,the chelating rate of ABMP-Cr(Ⅲ)was 90.13％.Agaricus blazei Murill polysaccharides(ABMP)had a long block structure,and the surface of ABMP-Cr(Ⅲ)was smooth and lamellar structure.The binding sites of Cr3+to polysaccharides were O-H,C-H and C-O-H.The hypoglycemic activity of ABMP-Cr(Ⅲ)increased with the increase of sample concentration,and its effect was better than that of ABMP.When ABMP-Cr(Ⅲ)was 0.6 mg·mL-1,the inhibition rate of α-amylase could reach 55.11％.When the concentration of ABMP-Cr(Ⅲ)was 5 mg·mL-1,the inhibition rate of α-glucosidase was 40.48％.[Conclusion]ABMP-Cr(Ⅲ)has high preparation efficiency under the optimal preparation conditions in this study,and its structure is significantly different from ABMP.ABMP-Cr(Ⅲ)has good hypoglycemic activity and has good development prospects.关键词
姬松茸/多糖-铬(Ⅲ)络合物/结构表征/降血糖活性Key words
Agaricus blazei/polysaccharide-chromium(Ⅲ)complex/chemical structure/hypoglycemic activity分类
医药卫生引用本文复制引用
林春林,李莹莹,刘朋虎,林冬梅,李晶,王义祥,陈华..姬松茸多糖-铬(Ⅲ)络合物的制备及降血糖活性体外评价[J].福建农业学报,2025,40(6):628-636,9.基金项目
国家重点研发计划项目(2023YFD1600501) （2023YFD1600501）
福建农林大学科技创新专项基金项目(KFB23191A) （KFB23191A）
福建省红壤山地农业生态过程重点实验室开放课题(Aephrs-202201) （Aephrs-202201）
