福建农业学报2025，Vol.40Issue(6)：637-648,12.DOI:10.19303/j.issn.1008-0384.2025.06.012
水肥一体化模式下施氮量对玉米根际土壤固氮菌群落及生长的调控
Effects of Nitrogen Application Rate on Rhizosphere Soil Nitrogen-Fixing Microbial Communities and Maize Growth under Integrated Irrigation-Fertilization Model
摘要
Abstract
[Objective]Effects of nitrogen(N)application rate on N-fixing microbial communities in rhizosphere soil and maize growth on land under integrated irrigation-fertilization model were analyzed.[Method]N-topdressing with no N(N0 as control),70%N at 98 kg·hm-2(N1),100%N at 140 kg·hm-2(N2),130%N at 182 kg·hm-2(N3)or 150%N at 210 kg·hm-2(N4)was applied on a maize growing field.High-throughput sequencing was conducted to compare the differences in the diversity and composition of N-fixing microbial communities in the rhizosphere soil.Growth of maize plants on the lots were correlated to the collected data on the soil at the same lot.[Result]Application of N fertilizer significantly increased the soil N as well as the maize biomass and N accumulation at growth stages.N2 yielded the optimal performance among all treatments.The α-diversity of N-fixing microbes in the rhizosphere soil decreased at first as the N application rate increased.It was followed by an increase and another decline.Of all treatments,N2 enhanced the α-diversity but N1 and N4 suppressed it.N-application significantly affected the soil microbial community structure,particularly during the jointing and flowering stages of maize.It also significantly raised the abundance of beneficial species,such as Bradyrhizobium.Notably,N2 enriched the relative abundance of plant growth-promoting bacteria of the unclassified order Burkholderiales.The upregulated modularity index and other topological parameters of the network analysis suggested a more stable community structure under N2 as well.The partial least squares path modeling further indicated that the rate of N-application indirectly stimulated the maize growth by way of altering the N composition in soil.[Conclusion]The N-application at a rate of 140 kg·hm-2(N2)appeared to optimize the soil N,stabilize the N-fixing microbial community,and promote the maize growth.Consequently,it was recommended for maize farming under the integrated irrigation-fertilization practice.关键词
玉米/水肥一体化/施氮量/根际土壤/固氮菌群落Key words
maize/integrated irrigation-fertilization/nitrogen application rate/rhizosphere soil/nitrogen-fixing microbial community分类
农业科技引用本文复制引用
徐莹莹,王宇先,高盼,杨慧莹,张巩亮,申惠波,刘玉涛..水肥一体化模式下施氮量对玉米根际土壤固氮菌群落及生长的调控[J].福建农业学报,2025,40(6):637-648,12.基金项目
黑龙江省农业科技创新跨越工程基础创新优青项目(CX23YQ20) （CX23YQ20）
中国科学院张兴义土壤学科学家工作室补助项目(202001) （202001）
齐齐哈尔市科技计划创新激励项目(CNYGG2023023) （CNYGG2023023）
