黑龙江八一农垦大学学报2025，Vol.37Issue(5)：10-17,8.DOI:10.3969/j.issn.1002-2090.2025.05.002
铁苋菜内生拮抗真菌鉴定及其抑菌活性分析
Identification and Antibacterial Activity Analysis of Endophytic Antagonistic Fungi in Acalypha Australis
吕清晗 1吴皓菘 1李明睿 1李宝成 1范文艳 1于军华 2姜述君1
作者信息
- 1. 黑龙江八一农垦大学农学院,大庆 163319
- 2. 黑龙江农垦红兴隆管理局农业发展部
- 折叠
摘要
Abstract
To identify the characteristics and biocontrol potential of the endophytic strain TA-1.3 of Acalypha australis,the taxonomic status and biological characteristics of the strain TA-1.3 was determined through morphological observation,ITS(Internal Transcribed Spacer)identification technology and adaptability of the strain to environmental factors.The inhibitory effects of the endophytic strain on Rhizoctonia solani AG1-IA,R.solani AG5,Sclerotinia sclerotiorum(Lib)de Bary,S.minor Jagger and Ma-gnaporthe oryzea were evaluated using the plate confrontation method and bacteriostatic secondary metabolites.Morphological observation shows that the colony of strain TA-1.3 emits coconut fragrance,the conidia pile is in concentric circular pattern,and the conidia stem is in pyramid shape,which has the typical morphological characteristics of Trichoderma.Through ITS identification and sequence alignment,the endophytic strain TA-1.3 was identified as Trichoderma harzianum.T.harzianum TA-1.3 had the fastest growth rate on media with starch as carbon source and yeast extract as nitrogen source,respectively.It was suitable for strain TA-1.3 cultivation in a dark environment,with an optimal pH of 9 and an optimal culture temperature of 25℃.The results of the confrontation test showd that the antagonism coefficient of T.harzianum TA-1.3 against AG1-IA,AG5,S.minor and M.oryzea all reached grade I.In a metabolite bacteriostatic test,the volatile metabolites of T.harzianum TA-1.3 produced the most obvious inhibition rate on S.sclerotiorum at 82.11%.The non-volatile metabolites of T.harzianum TA-1.3 had the most significant inhibitory effect on the growth of S.minor mycelium.关键词
铁苋菜/哈茨木霉/植物内生真菌/抑菌作用/病原真菌Key words
Acalypha australis/Trichoderma harzianum/endophytic fungi in plants/antibacterial effect.pathogenic fungi分类
农业科技引用本文复制引用
吕清晗,吴皓菘,李明睿,李宝成,范文艳,于军华,姜述君..铁苋菜内生拮抗真菌鉴定及其抑菌活性分析[J].黑龙江八一农垦大学学报,2025,37(5):10-17,8.
