黑龙江八一农垦大学学报2025，Vol.37Issue(5)：18-24,80,8.DOI:10.3969/j.issn.1002-2090.2025.05.003
一株莠去津降解菌的分离鉴定及降解性能研究
Isolation,Identification and Degradation Performance of a Strain of Atrazine Degrading Bacteria
摘要
Abstract
To further investigate the degradation process of atrazine and effectively remove the residual atrazine in the environment.A degrading strain named G1-N1A was isolated from the soil of the farmland where atrazine was applied for a long time in Xingan Co-unty by enrichment method and streak separation method.The strain was identified by morphological,physiological and biochemical characteristics and 16S rRNA sequence analysis.Degradation conditions were analyzed by single variable method.Degradation genes were detected by PCR,and metabolic pathways of atrazine were predicted by non-target metabolomics analysis.The results showed that strain G1-N1A was identified as Paenarthrobacter nitroguajacolicus,and its degradation rate of 50 mg·L-1 atrazine was 98.60%within 18 h.There was no significant difference in degradation rate in the range of inoculation dosage of 1%to 11%,temperature of 25 to 35℃,pH of 5 to 8,rotational speed of shaker of 50 to 200 r·min-1,and the degradation effect of 20 to 250 mg·L-1 was good.The strain contains trzN,atzB and atzC genes,and can degrade atrazine to cyanuric acid.G1-N1A provided strain resources for atrazine degradation in northern cold areas,and had a good application prospect in bioreremediation.关键词
莠去津/硝基癒疮木胶节杆菌/降解条件/生物修复Key words
atrazine/Paenarthrobacter nitroguajacolicus/degradation condition/bioremediation分类
资源环境引用本文复制引用
高涵,刘明,高梓峰,王昕,依桂华,郭永霞..一株莠去津降解菌的分离鉴定及降解性能研究[J].黑龙江八一农垦大学学报,2025,37(5):18-24,80,8.基金项目
黑龙江八一农垦大学三横三纵支持计划(ZRCPY201820) （ZRCPY201820）
黑龙江八一农垦大学科研启动计划(XDB201817) （XDB201817）
黑龙江省"双一流"新一轮建设学科协同创新成果建设项目(LJGXCG2022-107). （LJGXCG2022-107）
