华中农业大学学报2025，Vol.44Issue(5)：50-59,10.DOI:10.13300/j.cnki.hnlkxb.2025.05.005
杂交玉米苗期耐铝毒的多元综合评价及指标筛选
Comprehensive multivariate evaluation and screening of indicators for aluminum toxicity tolerance in hybrid maize seedlings
摘要
Abstract
To systematically evaluate the Al-toxicity of maize varieties and to establish an efficient screening system,45 hybrid maize varieties were selected to conduct Al-toxicity stress trials in this study.The experiment was designed in a dual-treatment mode:basic acidic soil(pH 5.15)and high-aluminum-toxic acidic soil to reveal the resistance mechanism of the varieties by comparing the differences in stress.During the critical growth stage of maize at the 3-leaf stage,the research team systematically measured 12 morpho-physiological indexes,including stem diameter,plant height,and SPAD value,and quantified the level of resistance by introducing the aluminum toxicity tolerance coefficient(CAlt).In this study,we used a multidimensional analysis method to construct an evaluation system,firstly,we extracted the characteristic indexes by dimensional reduction of principal component analysis,combined with the affiliation function method to calculate the comprehensive resistance evaluation value(D value),and then we used the system-atic clustering to classify the varieties into five resistance levels(among which extremely resistant D≥0.75,extremely sensitive D≤0.25),and then we screened out the extremely resistant varieties of aluminum toxic-ity,such as GY14022,EHD1,QKY900,and HY1702,and the aluminum toxicity resistant varieties of GY14022,EHD1,QKY900 and HY1702,and the aluminum toxicity resistant varieties of GY14022,EHD1,QKY900 and HY1702.HY1702,and extremely sensitive varieties TR1818,JF86 and ZY303.It was found that aluminum stress significantly inhibited the development of maize seedlings(P<0.001),the plant height and biomass decreased by 5.37%and 17.65%,respectively,and there was a significant correlation between the resistance indexes(P<0.05),which verified the reliability of the evaluation system.Finally,through the double validation of gray correlation and stepwise regression,the five core screening indexes of root fresh weight,total dry weight,SPAD value,total fresh weight and root length were confirmed.In con-clusion,aluminum toxicity stress severely inhibited the growth of maize at seedling stage,and there were significant differences in acid tolerance among different maize varieties.The comprehensive analysis screened root fresh weight,total dry weight,SPAD,total fresh weight and root length as the key indexes for screen-ing of aluminum toxicity resistance of maize at seedling stage.关键词
杂交玉米/铝毒胁迫/主成分分析/隶属函数法/聚类分析/逐步回归分析Key words
hybrid maize/aluminum toxicity stress/principal component analysis/affiliation function method/cluster analysis/stepwise regression analysis分类
农业科技引用本文复制引用
陈兴华,夏雨昕,李家鑫,丁广大,徐芳森,王创..杂交玉米苗期耐铝毒的多元综合评价及指标筛选[J].华中农业大学学报,2025,44(5):50-59,10.基金项目
国家重点研发计划项目(2022YFD1900705-4) （2022YFD1900705-4）
