核农学报2025,Vol.39Issue(11):2321-2331,11.DOI:10.11869/j.issn.1000-8551.2025.11.2321
60Co-γ射线辐射对不同文冠果优系种子萌发及幼苗生长的诱变效应
Mutagenic Effects of 60Co-γ Ray Radiation on Seed Germination and Seedling Growth of Different Xanthoceras sorbifolium Superior Lines
摘要
Abstract
In order to explore the radiation mutagenesis effects of 60Co-γ ray on Xanthoceras sorbifolium,six Xanthoceras sorbifolium superior line seeds were irradiated with different doses(0-750 Gy)of 60Co-γ ray.The effects of radiation on seed germination,seedling growth,and physiological and biochemical indexes were investigated.The results showed that within a certain radiation dose range,60Co-γ ray radiation treatment promoted the germination,seedling emergence and growth of Xanthoceras sorbifolium seeds,while these three indexes were gradually declined as the radiation dose increased.After radiation treatment,the seedlings showed morphological variations in traits such as leaf shape,number of leaflets,and leaf color.Compared to the control,the variation rate under 150-450 Gy radiation dose treatment was higher and the variation types were more abundant.The antioxidant enzyme activity,malondialdehyde(MDA)and soluble protein content in the leaves showed a trend of first rising and then falling with increasing radiation dose,while the chlorophyll content and soluble sugar content showed a downward trend.60Co-γ ray radiation demonstrated obvious mutagenic effects on Xanthoceras sorbifolium seeds,with the optimal dose range preliminarily established at 301.10 and 594.96 Gy.These results provide a technical reference for mutation breeding and creating new germplasm of Xanthoceras sorbifolium.关键词
文冠果/60Co-γ射线/诱变效应/生理生化Key words
Xanthoceras sorbifolium/60Co-γ ray/mutagenic effects/physiology and biochemistry引用本文复制引用
岑国秀,陆璐,霍玉雪,王磊,吴丹,仝伯强,杨克强,赵永军..60Co-γ射线辐射对不同文冠果优系种子萌发及幼苗生长的诱变效应[J].核农学报,2025,39(11):2321-2331,11.基金项目
山东省自然资源厅科技创新平台开放课题"文冠果种质资源补充收集与评价利用",山东省重点研发计划(农业良种工程)项目(2020LZGC0904) (农业良种工程)