核农学报2025，Vol.39Issue(11)：2332-2341,10.DOI:10.11869/j.issn.1000-8551.2025.11.2332
转录组分析筛选调控小麦叶姿形成的关键基因
Transcriptome Analysis to Identify Key Genes Regulating Leaf Posture Formation in Wheat
摘要
Abstract
In order to elucidate the molecular mechanism of leaf posture and leaf angle formation in wheat,the phenotypic and agronomic traits of lam1,an erect leaf posture mutant obtained by EMS mutation,were characterized,cellular section observation and transcriptome sequencing analysis were peformed.The results showed that,compared to the wild-type(WT),lam1 exhibited no significant changes in plant height and panicle type,while flag leaf posture shifted from drooping to erect,accompanied with a significantly reduced leaf angle.The flag leaf length of lam1 was shortened,while leaf width slightly increased.And the grain length and 1000-grain weight were significantly lower than those of the WT.While photosynthetic parameters were largely unchanged,but chlorophyll content was significantly decreased.Semi-thin section observations revealed that the erect leaf posture of lam1 was primarily due to reduced cell division in the adaxial parenchyma of the pulvinus,leading to a shortened total length from the epidermis to the central vascular bundle.Additionally,increased number of sclerenchyma cells on the abaxial side contributed to enhanced mechanical support for the leaf.Transcriptome sequencing analysis revealed that differentially expressed genes(DEGs)were mainly enriched in pathways related to phytohormone biosynthesis and signaling,phenylpropanoid metabolism,secondary cell wall formation,starch and sucrose synthesis.Specifically,we identified 8 auxin biosynthesis and metabolism-related genes,3 auxin-responsive MYB transcription factors(TFs),6 jasmonate-related genes,12 lignin biosynthesis genes,4 bHLH TFs,and 1 NAC TF.Notably,auxin-related DEGs were the most abundant,while bHLH TFs showed the highest fold changes.These genes may affect cell proliferation and wall thickening in the pulvinus,playing crucial roles in regulating wheat pulvinus development and leaf angle formation.This study provides new insights into the molecular regulatory mechanisms of leaf posture and angle in wheat.关键词
小麦/叶姿突变体/表型鉴定/转录组Key words
wheat/leaf posture mutant/phenotypic identification/transcriptome引用本文复制引用
武银玉,张婷,曹亚萍,张建华..转录组分析筛选调控小麦叶姿形成的关键基因[J].核农学报,2025,39(11):2332-2341,10.基金项目
山西省基础研究计划(202203021222166),山西农业大学生物育种工程项目(YZGC016) （202203021222166）
