核农学报2025，Vol.39Issue(11)：2351-2360,10.DOI:10.11869/j.issn.1000-8551.2025.11.2351
苦荞TIFY基因家族分析及FtJAZ8的抗立枯丝核病功能研究
Analysis of the TIFY Gene Family in Tartary Buckwheat and Study on the Resistance Function of FtJAZ8 against R.solani
摘要
Abstract
The TIFY gene family is a group of plant-specific transcriptional regulators widely involved in various biological processes including growth and development,hormone signaling,and stress responses.Within this family,the JAZ subfamily plays a crucial role in the jasmonic acid(JA)signaling pathway.To gain a deeper understanding of the evolutionary patterns and functions of the TIFY family in tartary buckwheat(Fagopyrum tataricum),this study integrated whole-genome information and systematically identified 17 TIFY members.Phylogenetic analysis,conserved domain identification,cis-acting element prediction,chromosomal localization,and collinearity analysis revealed the diversity and conservation of TIFY members in terms of subfamily distribution,structural characteristics,and regulatory elements.Through genome-wide association study(GWAS),FtJAZ8 was identified to be significantly associated with resistance to R.solani.An over-expression vector of FtJAZ8 was constructed and transformed into Arabidopsis thaliana and tartary buckwheat hairy roots.In vitro inoculation assays and physiological measurements were conducted.The results showed that FtJAZ8-OE plants exhibited lower disease indices and malondialdehyde(MDA)contents under R.solani infection.In addition,DAB staining and the upregulation of the pathogenesis-related gene PR1 in leaves further confirmed enhanced defense capacity.Over-expression of FtJAZ8 in the hairy root system also significantly alleviated pathogen-induced cell damage,further validating its key role in regulating plant disease resistance.This study not only established a systematic framework for the TIFY gene family in tartary buckwheat and clarified the functional mechanism of FtJAZ8,but also provided theoretical basis and candidate gene resources for molecular breeding of disease-resistant buckwheat varieties.关键词
苦荞/TIFY基因家族/FtJAZ8/立枯丝核菌(Rhizoctonia solani)/抗病Key words
tartary buckwheat/TIFY gene family/FtJAZ8/R.solani/disease resistance引用本文复制引用
刘培林,李世娟,范昱,李伟,周美亮,邵美红..苦荞TIFY基因家族分析及FtJAZ8的抗立枯丝核病功能研究[J].核农学报,2025,39(11):2351-2360,10.基金项目
建德市人民政府与中国农业科学院作物科学研究所粮食产业高质量发展合作项目(20230629),河北省自然科学基金资助(C2023204097) （20230629）
