核农学报2025，Vol.39Issue(11)：2361-2373,中插1-中插4,17.DOI:10.11869/j.issn.1000-8551.2025.11.2361
阳春砂AOC基因家族的鉴定和表达分析
Identification and Expression Analysis of AOC Gene Family in Amomum villosum Lour
摘要
Abstract
Allene oxide cyclase(AOC),a key enzyme in jasmonic acid biosynthesis,regulates plant growth,development,and stress responses.In order to preliminarily identify and analyze the function and expression pattern of AOC gene family in the genome of A.villosum,in this study,three candidate AvAOC genes were identified from A.villosum.Their functions were characterized using bio-informatics approaches and enzyme-linked immunosorbent assay(ELISA),and their tissue-specific expression patterns were analyzed.Results revealed that A.villosum harbors three AOC candidate genes.The candidate AvAOC proteins are unstable,hydrophilic,and non-secretory,sharing conserved motifs.AvAOC1 and AvAOC3 were predicted to localize in chloroplasts.Phylogenetic analysis revealed that the three candidate AvAOC genes clustered into a clade with those orthologs of monocotyledonous plants Zea mays and Oryza sativa.The genes were unevenly distributed across different chromosomal regions.Heterologous expression of the three candidate AvAOC genes in Pichia pastoris was performed,and ELISA confirmed that the recombinant proteins could bind to purified plant AOC antibodies.The quantitative real-time PCR(qRT-PCR)analysis confirmed the transient expression of the three candidate AvAOC genes in tobacco.These findings indicate that the three candidate AvAOC genes belong to the AOC gene family.RNA-seq expression profiling revealed that the three AvAOC genes were expressed in various tissues of A.villosum,including leaves,stolons,pericarps,seed clusters,and flowers.Notably,AvAOC2 expression was strongly induced in leaves upon infection by Colletotrichum gloeosporioides.This study provides a foundation for further exploration of AOC gene functions in A.villosum.关键词
阳春砂/AOC基因家族/生物信息学/毕赤酵母/烟草瞬时表达Key words
Amomum villosum Lour./allene oxide cyclase(AOC)gene family/bioinformatics/Pichia pastoris/transient expression in tobacco引用本文复制引用
张玉仪,钟子充,谢涛,涂宇婷,陈镕瀚,严萍,汤丽云,何国振..阳春砂AOC基因家族的鉴定和表达分析[J].核农学报,2025,39(11):2361-2373,中插1-中插4,17.基金项目
2024年省级乡村振兴战略专项资金种业振兴行动项目(2024-440000-90060000-8796),云浮市科技计划项目(2023020206),阳江市省科技创新专项资金项目(SDZX2023037) （2024-440000-90060000-8796）
